Identification of bHLH Family Transcription Factors of Wheat and Expression Analysis under Salt Stress
Abstact Basic helix-loop-helix,or bHLH,is a major family of plant transcription factors that is involved in signal transduction,biosynthesis,abiotic stress response,and plant growth and development.Using the winter wheat cultivar'Kenong 199'as the experimental material,we systematically analyzed the expression characteristics of wheat bHLH transcription factor under salt stress using transcriptional sequencing and bioinformatics analysis to investigate the functions of common wheat bHLH transcription factors in response to salt stress.According to the findings,wheat contains 489 bHLH transcription factors spreading across 21 chromosomes.Following the construction of the phylogenetic tree,the bHLH family was further subdivided into nine subfamilies,with the Ⅵ subfamily having the greatest number of members and the Ⅳ subfamily having the least.Wheat roots'transcriptome sequencing revealed 44 differentially expressed genes(DEGs),of which 17 were up-regulated and 27 were down-regulated at one and six hours after salt stress.According to GO analysis,DEGs were more abundant in the regulation of auxin,water and salt stress.According to KEGG annotation analysis,DEGs were primarily enriched in metabolic pathways related to starch and sucrose,amino sugars,nucleotide sugars,and other signaling pathways.The accuracy of the RNA-Seq results was confirmed through qRT-PCR verification of a few DEGs,which revealed that their expression pattern matched the transcriptome sequencing results.
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