Effects of Uniconazole Combined with Melatonin on Germination and Root Morphology of Adzuki Bean
In order to improve the quality of seedlings by treating seeds with plant growth regulators,this study set the concentration of uniconazole at 2,4,8,16 and 32 mg/L,and the above concentration of uniconazole and 100 μmol/L melatonin compound coating treatment,with no drug solution coating as the control,to study the effects of different concentrations of uniconazole and its combination with melatonin on seed germination,root morphological characteristics,antioxidant enzyme activities and sugar contents of adzuki bean.The results showed that the germination index of adzuki bean treated with 8 mg/L uniconazole(S8)and its combination with melatonin(S8M)were significantly higher than that of the control,and the germination rate was not significantly different compared with control.With the increase of uniconazole concentration,the contents of fructose,soluble sugar and soluble protein in roots showed a trend of first up and then down,all of which were the highest in S8 treatment,while the contents of sucrose and starch in roots did not show obvious regularity.Compared with other treatments,S8 and S8M treatments were more conducive to maintaining a high level of antioxidant enzyme activities and significantly improving root activity.Correlation analysis showed that there were significant and extremely significant correlations between the above physiological indexes and root morphological indexes.Comprehensive analysis showed that 8 mg/L uniconazole coating and its combination with 100 μmol/L melatonin coating could promote the germination and root development of adzuki bean,which was conducive to the emergence of neat and strong seedlings.
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