江苏淮安市高粱上玉米黄花叶病毒的分子检测与鉴定
Molecular Detection and Identification of Maize Yellow Mosaic Virus on Sorghum in Huai'an,Jiangsu
王海涛 1任春梅 1董岩 1李硕 1程兆榜 1季英华1
作者信息
- 1. 江苏省农业科学院植物保护研究所/江苏省食品质量安全重点实验室/国家重点实验室培育基地,210014,江苏南京
- 折叠
摘要
为掌握江苏省高粱病毒病的发生情况,从江苏淮安采集高粱疑似病毒病样品20份,进行了分子检测与鉴定.结果发现,使用玉米黄花叶病毒(maize yellow mosaic virus,MaYMV)引物可扩增出与目的片段一致的条带,检出率为80％.利用MaYMV外壳蛋白(CP)基因引物,以阳性样本RNA为模板进行扩增、克隆、酶切验证和测序分析,并构建进化树发现,淮安分离物CP基因与江苏分离物CP基因的进化关系较近.这些结果表明江苏淮安高粱受到了 MaYMV侵染,这是该病毒在我国高粱上的首次报道.
Abstract
In order to understand the incidence of sorghum virus disease in Jiangsu province,the 20 suspected sorghum virus disease samples were collected from Huai'an city,and molecular detection and identification were carried out.The results showed that the bands consistent with the target fragment could be amplified by using the maize yellow mosaic virus(MaYMV)primer,and the detection rate was 80％.We used MaYMV coat protein(CP)gene primers to perform amplification,cloning,enzyme digestion and sequencing analysis using a positive RNA as template.The phylogenetic tree analysis showed that CP genes of Huai'an isolate and Jiangsu isolate had a close evolutionary relationship.These results indicated that this is the first report of MaYMV infection in China sorghum.
关键词
玉米黄花叶病毒/高粱病毒病/分子检测/进化树分析Key words
Maize yellow mosaic virus/Sorghum virus disease/Molecular detection/Phylogenetic tree analysis引用本文复制引用
基金项目
国家自然科学基金(32001871)
国家自然科学基金(32072506)
江苏省自然科学基金(BK20200283)
出版年
2024
国家科技期刊平台
NSTL
万方数据