Genetic Diversity and Comprehensive Evaluation of Phenotypic Traits of 162 Tartary Buckwheat Resources in Inner Mongolia
Genetic diversity index,coefficient of variation,correlation analysis,principal component analysis,cluster analysis and stepwise regression analysis were used to analyze and evaluate 13 phenotypic traits of 162 tartary buckwheat germplasm resources.The results showed that the genetic diversity index of five qualitative traits was 0.7180-1.2826,the genetic diversity index of eight quantitative traits were 1.3037-2.0727,the coefficients of variation were 5.4％-43.4％,and the coefficients of variation of the other seven agronomic traits were all greater than 10％except the growth period,which indicated that the genetic variation of selected tartary buckwheat resources was very rich.Correlation analysis showed that six characteristics,such as branch number of main stem,node number of main stem,seed number per plant,seed weight per plant,1000-grain weight and growth period,had the greatest influence on yield.The cumulative contribution rate of four principal components extracted by principal component analysis was 83.559％.The main factors that cause the phenotypic variation of tartary buckwheat were grain weight per plant,grain number per plant,yield,plant type,branch number of main stem and grain shape.The 162 tartary buckwheat germplasm resources were grouped into five groups by cluster analysis,among which the agronomic characteristics of the materials in group Ⅰ were relatively balanced,which was an ideal material for breeding excellent tartary buckwheat varieties(lines).The materials of group V had better lodging resistance and better performance,which was a better choice for lodging-resistant parents.Through fuzzy membership function,a comprehensive evaluation index was constructed based on the contribution rate weight of four principal components,and ten tartary buckwheat germplasm resources with better comprehensive characteristics were screened out.
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