Cloning,Bioinformatics and Expression Analysis of FtERF Gene in Fagopyrum tataricum
Ethylene response factor plays an important role in plant development and cell metabolism.ERF gene was cloned from Tartary buckwheat by RT-PCR,and its bioinformatics and differential expression were analyzed.The results showed that the length of open reading frame of FtERF gene was 729 bp,encoding 243 amino acids,molecular weight of 26.08 kD,isoelectric point of 9.22,belonging to the hydrophilic protein.FtERF didn't contain signal peptides and transmembrane helical structure,and was located in the nucleus of the cell.The secondary and tertiary structures of the proteins were highly similar.FtERF contained the inhibitory motif DLNxxP,and phylogenetic evolution suggested that FtERF and ERF4 were closely related.The results showed that the expression of Tartary buckwheat in different parts of thick-shell were higher than those in thin-shell Tartary buckwheat,and the expression of Tartary buckwheat in mature period was higher than that in immature period.
NETL
NSTL
万方数据
