Cloning and Expression Analysis of Potato StCWIN1 Gene Promoter and Its Role under Drought Stress
Cell wall invertase(CWIN)is a key enzyme for stress responses and sucrose metabolism in source and sink tissues.In this study,the promoter sequence of StCWIN1 was cloned by genomic walking,and its cis-regulatory elements were analysed by using PlantCARE.A GUS-fused StCWIN1 promoter expression vector was constructed and subsequently transformed into Arabidopsis thaliana.Promoter activity,tissue expression characteristics and responsiveness to drought stress were investigated by histochemical staining and real-time quantitative PCR.The results showed that the StCWIN1 promoter was 1956 bp long and contained enhancer-regulated,phytohormone-responsive,protective/stress-responsive and light-responsive elements.The expression activity of StCWIN1 promoter were higher in roots,stigmas,and pods than other tissues.The expression of GUS in leaves of Arbidopsis Thaliana with StCWIN1 promoter was higher than that of wild type,and the value was significantly reduced under drought stress.The identification of the active StCWIN1 promoter in the present study suggests that StCWIN1 may be involved in the development of organs such as roots,flowers and fruit pods,and may also function in response to drought stress.
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