Effects of Water Deficit in Soybean Seedling Stage on Soil Enzyme Activity and Microbial Diversity
In order to study the effects of different degrees of water deficit on soil enzyme activities and soil microbial diversity of soybean,a pot experiment was conducted with drought sensitive soybean Suinong 26.At seedling stage,soil water content was set at 70％(normal water supply,CK),60％(mild water deficit,T1),50％(moderate water deficit,T2)and 40％(severe water deficit,T3)of field capacity treatments,the soil enzyme activity was determined,and the high-throughput sequencing technique was used to study the community composition and diversity of soil microorganisms.The results showed that soil urease activity in T1 treatment was the highest in all growth stages except mature stage.Compared with CK,soil sucrase activity in different water deficit treatments decreased by 13.89％to 21.94％.After rehydration,soil catalase activity was the highest with T2 treatment,while soil phosphatase activity reached the highest with T1 treatment.Water deficit could lead to changes in the number of soil microorganisms,increase in richness,and there was a certain correlation with enzyme activities.The yield per plant of soybean reached the maximum with T1 treatment,which was significantly higher than that of CK by 9.55％.To sum up,moderate water deficit could promote soil enzyme activity to a certain extent,and further affected soil microenvironment,so as to achieve the purpose of saving water and increasing production.
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