Screening and Feature Analysis of Atoxigenic Aspergillus flavus in Reducing Aflatoxin Prodution in Peanuts Soil in Xiangyang
Several Aspergillus flavus strains were isolated from Xiangyang,Hubei peanut soil medium.Through characteristic analysis and sequencing identification,the trains were identified as Aspergillus flavus strains.Using liquid fermentation culture,aflatoxin content in the soil was detected,and the atoxigenic Aspergillus flavus strains were obtained at the same time.Through the flat screening,the several good Aspergillus flavus strains were screened at the beginning.The standard and non-virulent strains were used in symbiotic culture to determine the AFT content.The results showed that there were four strains of enterotoxigenic symbiotic strains of Aspergillus flavus,toxins aflatoxin content lower than control strains over 80％,including XZ38 inhibitory reached 99.49％.This indiated that non-toxigenic Aspergillus flavus strains had a great role in the inhibition of enterotoxigenic aspergillus flavus growth,reduction or elimination aflatoxin,and could be used as alternative strains for biological control for the field aflatoxin contamination offers a good alternative.
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