Transcriptome Analysis of Different Foxtail Millet(Setaria italica L.)Varieties Treated with Imazapic Herbicide
Imidazolinone herbicides can effectively control monocotyledonous and dicotyledonous weeds in foxtail millet fields.In order to explore the molecular mechanism of millet resistance to imidazolinone herbicides,after the emergence of resistant varieties(R)and sensitive varieties(S)differentially expressed genes and metabolic pathways in resistant and susceptible foxtail millet cultivars were analyzed by high-throughput RNA-Seq sequencing after 15 d of uniform spraying with methamphetamine nicotinic acid.The results showed that genes related to photosynthesis and metabolic pathways were down-regulated after herbicide treatment in both cultivars.However,the down-regulation was more significant in the sensitive cultivar.Similarly,fatty acid elongation genes were significantly down-regulated after herbicide treatment in the sensitive cultivar.Quantitative real-time PCR(qRT-PCR)results of five candidate genes showed excellent agreement with those deep sequencing.These genes may play an important role in the imidazolinone herbicide resistance.
NETL
NSTL
万方数据
