不同谷子品种喷施咪唑啉酮除草剂后的转录组分析
Transcriptome Analysis of Different Foxtail Millet(Setaria italica L.)Varieties Treated with Imazapic Herbicide
宋慧 1王涛 2邢璐 1刘俊芳 1张扬 1刘金荣 1陈红旗 1冯佰利3
作者信息
- 1. 安阳市农业科学院,455000,河南安阳
- 2. 安阳工学院生物与食品工程学院,455000,河南安阳
- 3. 西北农林科技大学农学院/旱区作物逆境生物学国家重点实验室,712100,陕西杨凌
- 折叠
摘要
咪唑啉酮类除草剂能有效防治谷子田的单、双子叶杂草,为探究谷子对咪唑啉酮类除草剂抗性的分子机制,于抗性品种(R)和敏感品种(S)出苗后15d均匀地喷洒甲咪唑烟酸,通过高通量RNA-Seq测序分析抗性和敏感谷子品种中差异表达的基因和代谢途径.结果表明,2个品种在除草剂处理后,与光合作用和代谢途径相关的基因均被下调,特别是在敏感品种中下调更为显著.同样,敏感品种在除草剂处理后,与脂肪酸延伸相关基因表达也显著下调.5个随机选择基因的实时定量PCR(qRT-PCR)的结果与深度测序的结果一致.上述相关基因可能在谷子对咪唑啉酮类除草剂的抗性中起重要作用.
Abstract
Imidazolinone herbicides can effectively control monocotyledonous and dicotyledonous weeds in foxtail millet fields.In order to explore the molecular mechanism of millet resistance to imidazolinone herbicides,after the emergence of resistant varieties(R)and sensitive varieties(S)differentially expressed genes and metabolic pathways in resistant and susceptible foxtail millet cultivars were analyzed by high-throughput RNA-Seq sequencing after 15 d of uniform spraying with methamphetamine nicotinic acid.The results showed that genes related to photosynthesis and metabolic pathways were down-regulated after herbicide treatment in both cultivars.However,the down-regulation was more significant in the sensitive cultivar.Similarly,fatty acid elongation genes were significantly down-regulated after herbicide treatment in the sensitive cultivar.Quantitative real-time PCR(qRT-PCR)results of five candidate genes showed excellent agreement with those deep sequencing.These genes may play an important role in the imidazolinone herbicide resistance.
关键词
咪唑啉酮/转录组/差异表达基因/谷子Key words
Imazapic/Transcriptome/Differentially expressed genes/Foxtail millet引用本文复制引用
基金项目
国家现代农业产业技术体系建设专项(CARS-06-14.5-B25)
河南省青年人才托举工程项目(2021HYTP035)
河南省现代农业产业体系建设专项(HARS-22-04-Z1)
河南省自然科学基金(222300420101)
出版年
2024
国家科技期刊平台
NSTL
万方数据