Genomic SSR Loci Mining and Genetic Diversity Analysis of Camellia oleifera Based on Genome Sequences
SSR(Simple Sequence Repeat)loci were excavated from the whole genome sequence of Camellia oleifera Abel available in the NCBI database.Primer pairs were designed using Primer 3.0 software,and those with high polymorphism were selected for genetic diversity evaluation.A total of 1 661 881 SSR loci were detected with a cumulative length of 2 889 508 820 bp,occurring at a frequency of 63.22％.Mono-nucleotide repeats were the most abundant(61.72％),followed by di-nucleotide repeats(28.59％)and tri-nucleotide repeat units(6.90％).Analysis of repeat sequence motifs revealed the presence of 337 types of repeat elements,with A/C being the predominant repeat unit,followed by A/T(60.46％),AG/TC(13.88％),AT/TA(12.09％),AC/TG(2.86％),and AAT/TTA(2.54％).The 10 pairs of primers per chromosome(a total of 150 pairs of primers)were synthesized and assessed for polymorphism.The results indicated that 129 primer pairs successfully amplified target bands,with 25 primer pairs displaying specificity among different materials.The utilization of the whole genome sequence of C.oleifera enabled the large-scale development of SSR markers,which could be employed for genetic diversity analysis,construction of genetic maps,variety identification,and other related research in C.oleifera.
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