Effects of Compound Microbial Fertilizer on Drought Resistance of Maize Seedlings under Drought Stress by Transcriptome Analysis
To explore the effects of compound microbial fertilizer on drought resistance of maize seedlings under drought stress,four treatments,blank control group(CK),phosphate solubilizing bacteria addition group(P),arbuscular mycorrhizal fungi addition group(A)and compound microbial fertilizer addition group(including phosphate solubilizing bacteria and arbuscular mycorrhizal fungi)(AP),were set up respectively with maize"Eyu 16"as the material,and then subjected to drought stress treatment through soil water control.Maize leaf samples were collected for physiological and biochemical index detection and transcriptome sequencing analysis.The results showed that after drought stress treatment(i.e.the fifth,tenth and fifteenth days),the highest MDA contents in maize leaves was CK,the lowest was AP treatment,and the activities of SOD and POD in maize leaves in AP treatment were significantly higher than those in CK,and the activities of CAT in maize leaves in AP treatment were higher than those in CK treatment during stress.Compared with CK,transcriptome sequencing analysis detected 320 differentially expressed genes(DEGs)in the leaves of maize seedlings in AP treatment,including 204 up-regulated genes and 116 down-regulated genes;the results of GO analysis showed that 34.33%,43.12%and 22.55%of DEGs were enriched in biological processes,cell components and molecular functions,respectively.The results of KEGG analysis showed that the significant enrichment pathways included plant pathogen interaction,starch and sucrose metabolism,other types of O-glycan biosynthesis,zeatin biosynthesis,galactose metabolism and amino acid biosynthesis.