Analysis of Genetic Diversity and Genetic Distance of Maize Inbred Lines Based on Phenotypic Traits of Husks
In order to accurately identify the genetic basis of maize inbred line germplasm resources and explore the distance of genetic relationships between germplasm resources,42 maize backbone inbred lines were used as experimental materials,and genetic diversity and genetic distance of maize inbred line germplasm resources were studied through genetic variation,principal component analysis,and genetic distance clustering analysis based on eight husk phenotypic traits.The results showed that the variation range of husk phenotypic traits of 42 maize inbred line germplasm resources was 3.23%-26.15%,and the average coefficient of variation was 17.03%.The variation of husk dry weight and the longest husk width were abundant,but the variation of husk tightness was the least.The genetic diversity index(H')ranged from 1.53 to 1.99,the genetic diversity index for husk dry weight was the highest,while the husk layer was the lowest.Correlation analysis showed that there were complex correlations among the husk phenotypic traits of inbred lines.There was a positive correlation between husk dry weight and husk layer,husk length,husk width,and husk thickness,while husk layer was significantly negatively correlated with husk length and width.The differences in particular factor qualities of 42 maize backbone inbred lines were examined,and the first three principal component scores served as markers for the two-dimensional sorting of principal components.The principal component analysis results showed that the cumulative contribution rate of the first three principal components reached 74.21%.Euclidean distance clustering analysis showed that 42 maize inbred lines were divided into five groups,and the average genetic distance between groups was 10.64.