Effects of Film Mulching on Rhizosphere Soil Microbial Community of Maize in Wind-Sand Grassy Beach Area of Northern Shaanxi Province
In order to clarify the changes of microbial community structure in maize rhizosphere soil under different mulching conditions,six samples of maize rhizosphere soil under different mulching conditions were collected.High-throughput sequencing technology was used to sequence the gene regions of microbial samples of maize rhizosphere soil,so as to analyze the community structure characteristics of bacteria and fungi in the sample rhizosphere soil,and reveal the influence of mulching soil on the quality of maize rhizosphere soil.The results showed that the total OTU number of bacteria and fungi in the field covered with mulch were higher than that in the field without mulching.Chaol and Shannon indexes revealed that the richness and diversity of fungi and bacteria community in the rhizosphere soil of maize under mulching were higher than those without mulching.At the phylum level,the relative abundance of Proteobacteria and Bacteroidota were decreased and that of Acidobacteria,Gemmatimonadota,Ascomycota and Glomeromycota were increased.At the genus level,film mulching caused the enrichment of MND1,Haliangium,Metagenome,RB41,Sphingomonas in bacteria and Exophiala,Metarhizium,Botryotrichum,Coniochaeta,Lophotrichus,Fusarium in fungi in the rhizosphere soil of maize.This study presented the scientific theoretical foundation for the selection,use,and exploitation of beneficial microorganisms in maize production.It also demonstrated the impact of agricultural mulching on the ecological effect of maize rhizosphere soil.
Film mulchingMaize rhizosphere soilHigh-throughput sequencingMicrobial community