Identification and Evaluation of Drought Tolerant Germplasm Resources at Seedling Stage of Maize under PEG Stress
In order to establish an evaluation system of maize drought tolerance at seedling stage and screen excellent drought tolerant maize germplasm resources,124 maize inbred lines commonly used in Northeast China were used as test materials for hydroponics at seedling stage,and 20%PEG-6000 was used to simulate drought.The drought-stress tolerance index of inbred lines was calculated by measuring plant height,stem diameter,root length,fresh weight aboveground,fresh weight underground,plant water content and chlorophyll relative content.Principal component analysis,regression analysis and cluster analysis were used to evaluate drought tolerance of maize materials.The results showed that chlorophyll,fresh weight underground and stem diameter could be used as the first choice indicators for screening drought tolerance of maize seedlings,and maize seedlings could be divided into four categories,including drought tolerance,medium resistance,drought sensitive and highly sensitive inbred lines.Drought tolerance inbred lines included nine inbred lines,accounting for 7.26%of the total number of materials.There were 47 moderately resistant inbred lines,accounting for 37.90%;there were 52 drought sensitive inbred lines,accounting for 41.94%;there were 16 highly sensitive inbred lines,accounting for 12.90%of the total material.
MaizeDroughtGermplasm resourcesEvaluation of drought tolerance