PEG胁迫下玉米苗期耐旱种质资源鉴定与评价
Identification and Evaluation of Drought Tolerant Germplasm Resources at Seedling Stage of Maize under PEG Stress
王璐 1邓杰 1张泽 1赵孟伟 1车欣洋 1王广义 1郭旭 1张海洋 1贺琳 1翁建峰 2徐晶宇1
作者信息
- 1. 黑龙江八一农垦大学农学院/黑龙江省现代农业栽培技术与作物种质改良重点实验室,163319,黑龙江大庆
- 2. 中国农业科学院作物科学研究所,100081,北京
- 折叠
摘要
为建立玉米苗期耐旱能力评价体系,筛选优良的耐旱玉米种质资源,以东北地区常用的124份玉米自交系为供试材料进行苗期水培,利用20％PEG-6000模拟干旱,测定干旱胁迫下自交系株高、茎粗、根长、地上鲜重、地下鲜重、植株含水量、叶绿素相对含量等10个性状,计算各指标耐旱胁迫指数.利用综合隶属函数法,进行主成分分析、回归分析和聚类分析,综合评价各玉米材料耐旱能力.结果表明,叶绿素相对含量、地下鲜重、茎粗可作为玉米苗期耐旱能力筛选的首选指标,并根据苗期耐旱能力将自交系分为4类,分别为旱耐受、中抗性、旱敏感、极敏感;旱耐受自交系共9个,占材料总数的7.26％;中抗性自交系47个,占比37.90％;旱敏感自交系52个,占比41.94％;极敏感自交系16个,占比12.90％.
Abstract
In order to establish an evaluation system of maize drought tolerance at seedling stage and screen excellent drought tolerant maize germplasm resources,124 maize inbred lines commonly used in Northeast China were used as test materials for hydroponics at seedling stage,and 20％PEG-6000 was used to simulate drought.The drought-stress tolerance index of inbred lines was calculated by measuring plant height,stem diameter,root length,fresh weight aboveground,fresh weight underground,plant water content and chlorophyll relative content.Principal component analysis,regression analysis and cluster analysis were used to evaluate drought tolerance of maize materials.The results showed that chlorophyll,fresh weight underground and stem diameter could be used as the first choice indicators for screening drought tolerance of maize seedlings,and maize seedlings could be divided into four categories,including drought tolerance,medium resistance,drought sensitive and highly sensitive inbred lines.Drought tolerance inbred lines included nine inbred lines,accounting for 7.26％of the total number of materials.There were 47 moderately resistant inbred lines,accounting for 37.90％;there were 52 drought sensitive inbred lines,accounting for 41.94％;there were 16 highly sensitive inbred lines,accounting for 12.90％of the total material.
关键词
玉米/干旱/种质资源/耐旱性评价Key words
Maize/Drought/Germplasm resources/Evaluation of drought tolerance引用本文复制引用
基金项目
黑龙江省自然基金重点项目(ZD2020C007)
黑龙江八一农垦大学三横三纵重点专项(ZDZX202101)
出版年
2024
国家科技期刊平台
NSTL
万方数据