复合微生物肥对棉田土壤养分及根际细菌群落的影响
Effects of Compound Microbial Fertilizer on Soil Nutrients and Rhizosphere Bacterial Community in Cotton Field
吕博 1丁亮 1过聪 1陈锋 1周海平 2汪雪松 2董小林 3向发云1
作者信息
- 1. 湖北省农业科学院经济作物研究所/农业农村部长江中游棉花生物学与遗传育种重点实验室,430072,湖北武汉
- 2. 鄂州市农业科学研究所,436000,湖北鄂州
- 3. 农业农村部长江中游作物绿色高效生产重点实验室/长江大学农学院,434025,湖北荆州
- 折叠
摘要
为探究复合微生物肥对棉田土壤养分及根际细菌群落的影响,设置不施用复合微生物肥(T0)和施用复合微生物肥(T1～T4)共5个处理,通过田间试验测定各处理棉花根际土壤养分含量、棉花籽棉和皮棉产量,并对不同处理根际土样本进行16S rDNA高通量测序分析.结果表明,与T0相比,T1～T4处理的土壤有机质、碱解氮、有效磷和速效钾含量显著增加,籽棉产量提高5.59％～14.28％,皮棉产量提高5.65％～16.64％.各处理土壤中优势菌门主要为变形菌门(Proteobacteria)和酸杆菌门(Acidobacteria),优势菌目主要为鞘脂单胞菌目(Sphingomonadales)、酸杆菌目(Acidobacteriales)、芽单胞菌目(Gemmatimonadales).与 T0 相比,T1～T4处理土壤中鞘氨醇单胞菌属(Sphingomonas)和芽单胞菌属(Gemmatimonas)相对丰度显著升高,而酸杆菌属(Acidobacterium)和RB41相对丰度显著降低.鞘氨醇单胞菌属和芽单胞菌属相对丰度与有机质、碱解氮、有效磷、速效钾含量呈正相关.综上所述,施用复合微生物肥可增加棉花根际优势菌群鞘氨醇单胞菌属和芽单胞菌属相对丰度,促进土壤养分释放,提高棉花产量.
Abstract
In order to explore the effects of compound microbial fertilizer on soil nutrients and rhizosphere bacterial community in cotton field,five treatments of without application of compound microbial fertilizer(T0)and application of compound microbial fertilizer(T1-T4)were set up.Field experiments were conducted to determine the nutrient content of cotton rhizosphere soil,cotton seed and lint yield under different treatments,and high-throughput 16S rDNA sequencing was performed on rhizosphere soil samples from different treatments.The results showed that compared with T0,the contents of soil organic matter,alkaline nitrogen,available phosphorus and available potassium in T1-T4 treatments increased significantly,the seed cotton yield increased by 5.59％-14.28％,and the lint yield increased by 5.65％-16.64％.The dominant bacteria in different soil treatments at phylum level were mainly Proteobacteria and Acidobacteria,while the dominant bacterial in different treatments at the order level were mainly Sphingomonadales,Acidobacteriales and Gemmatimonadales.Compared with the control,the relative abundance of Sphingomonas and Gemmatimonas in the soil treated with T1-T4 increased significantly,while the relative abundance of Acidobacterium and RB41 decreased significantly.The relative abundance of Sphingomonas and Gemmatimonas were positively correlated with the contents of organic matter,alkaline nitrogen,available phosphorus and available potassium.In summary,the application of compound microbial fertilizer can increase the abundance of the dominant rhizosphere bacteria of cotton,Sphingomonas and Gemmatimonas,promote the release of soil nutrients,and increase cotton yield.
关键词
复合微生物肥/棉花/产量/土壤养分/细菌群落Key words
Compound microbial fertilizer/Cotton/Yield/Soil nutrients/Bacterial communities引用本文复制引用
基金项目
湖北省农业科技创新中心项目(2019-620-000-001-08)
出版年
2024
国家科技期刊平台
NSTL
万方数据