Effects of Compound Microbial Fertilizer on Soil Nutrients and Rhizosphere Bacterial Community in Cotton Field
In order to explore the effects of compound microbial fertilizer on soil nutrients and rhizosphere bacterial community in cotton field,five treatments of without application of compound microbial fertilizer(T0)and application of compound microbial fertilizer(T1-T4)were set up.Field experiments were conducted to determine the nutrient content of cotton rhizosphere soil,cotton seed and lint yield under different treatments,and high-throughput 16S rDNA sequencing was performed on rhizosphere soil samples from different treatments.The results showed that compared with T0,the contents of soil organic matter,alkaline nitrogen,available phosphorus and available potassium in T1-T4 treatments increased significantly,the seed cotton yield increased by 5.59％-14.28％,and the lint yield increased by 5.65％-16.64％.The dominant bacteria in different soil treatments at phylum level were mainly Proteobacteria and Acidobacteria,while the dominant bacterial in different treatments at the order level were mainly Sphingomonadales,Acidobacteriales and Gemmatimonadales.Compared with the control,the relative abundance of Sphingomonas and Gemmatimonas in the soil treated with T1-T4 increased significantly,while the relative abundance of Acidobacterium and RB41 decreased significantly.The relative abundance of Sphingomonas and Gemmatimonas were positively correlated with the contents of organic matter,alkaline nitrogen,available phosphorus and available potassium.In summary,the application of compound microbial fertilizer can increase the abundance of the dominant rhizosphere bacteria of cotton,Sphingomonas and Gemmatimonas,promote the release of soil nutrients,and increase cotton yield.
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