Effects of Biochar and Nitrogen Fertilizer on the Physiological Growth Characteristics of Quinoa Seedlings under Saline Alkali Stress
To explore the effects of biochar and nitrogen fertilizer on the physiological growth characteristics of quinoa seedlings under saline-alkali stress,a study conducted a pot experiment to analyze various physiological indices such as chlorophyll content,antioxidant enzyme activity,and osmotic regulation substances of Longli 1 seedlings.A randomised block experiment was conducted with four levels of biochar[0 B0),5(B1),15(B2),25(B3)g/kg]and three levels of nitrogen fertilizer[0.09(Nl),0.15(N2),0.21(N3)g/kg],and without biochar and nitrogen fertilizer as a control(CK).The results showed that in the absence of biochar,as the nitrogen application rate increased,plant height and main root length of quinoa seedlings increased,whereas the stem diameter initially increased and then decreased.Under the same nitrogen application rate,as the biochar application rate increased,relative water content,chlorophyll content,and antioxidant enzyme activity of quinoa seedlings initially increased and then decreased;whereas malondialdehyde(MDA),soluble sugar content,and proline content increased initially and then decreased.Under the same biochar application rate,with the increasing of nitrogen application rate,the relative water content,chlorophyll content,and antioxidant enzyme activity of quinoa seedlings initially increased and then decreased,while the MDA,soluble sugar,and proline contents initially decreased and then increased,and they were all best under N2 treatment.The results of subordination function showed that N2B2 was the most effective treatment for mitigating saline-alkali stress of quinoa seedlings.In conclusion,applying biochar and nitrogen fertilizer significantly promoted the growth and physiological characteristics of quinoa seedlings under saline-alkali stress,achieving the best effect with 15 g/kg biochar and 0.15 g/kg nitrogen fertilizer.
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