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不同玉米品种抗旱性的生理鉴定与综合评价

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在新疆吐鲁番地区以42个玉米主栽品种为材料,设置正常灌溉、中度干旱和重度干旱处理,测定玉米灌浆期穗位叶叶绿素(Chl)、丙二醛(MDA)和脯氨酸(Pro)含量,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活性以及籽粒产量.采用灰色关联度分析、抗旱指数(DI)、抗旱性度量值(D值)、加权抗旱指数(WDI))和聚类分析相结合的方法,筛选与抗旱紧密相关的生理指标,并进行综合评价.结果表明,中度干旱胁迫下,上述6个生理指标与综合抗旱指数(CDI)的关联度依次为SOD(0.803)、Chl(0.796)、MDA(0.788)、CAT(0.770)、Pro(0.756)、POD(0.755);重度干旱胁迫下,这 6 个生理指标与 CDI的关联度依次为 SOD(0.725)、MDA(0.724)、Chl(0.718)、Pro(0.692)、CAT(0.681)、POD(0.667).基于D值和WDI值的42个玉米品种抗旱性评价结果存在一定差异,但不同品种抗旱性排序基本一致.相关性分析表明,产量DI与综合抗旱性度量值(CD)和综合加权抗旱指数(CWDI)均呈显著正相关.根据CWDI将42个玉米品种抗旱性分为高抗、中抗、低抗,其中高抗品种2个,中抗品种13个,低抗品种27个.方差分析表明,高抗玉米组产量DI显著高于中抗和低抗.综上,以上玉米灌浆期穗位叶片6个生理指标可作为玉米品种抗旱性生理鉴定的参考指标,42个参试品种中辽单145和锦华150具有较强抗旱性.
Physiological Identification and Comprehensive Evaluation of Drought Resistance of Different Maize Varieties
A total of 42 maize cultivars were selected as the test materials and treated with normal irrigation,moderate and severe drought stress in the experimental base of Turpan Institute of Agricultural Sciences,Xinjiang Academy of Agricultural Sciences.The changes of chlorophyll(Chi)content,malondialdehyde(MDA)content,proline(Pro)content,the activities of superoxide dismutase(SOD),catalase(CAT)and peroxidase(POD)in ear leaf of maize at grain filling stage,and also grain yield were measured.The grey correlation analysis,drought resistance index(DI),drought resistance evaluation values(D-value),weighted drought resistance index(WDI-value)and cluster analysis were used to screen physiological indexes closely related to drought resistance,and the drought resistance of different maize varieties was evaluated comprehensively.The results showed that the correlation degree between the six physiological indexes and the comprehensive drought resistance index(CDI)under moderate drought stress were SOD(0.803),Chi(0.796),MDA(0.788),CAT(0.770),proline(0.756)and POD(0.755);Under severe drought stress,the correlation degree between the six physiological indexes and the CDI were SOD(0.725),MDA(0.724),Chl(0.718),proline(0.692),CAT(0.681)and POD(0.667).Based on the D value and WDI value,the drought resistance evaluation results of the two methods had some differences,but the drought resistance ranking of different varieties was basically the same.The correlation analysis of average DI of yield and values of(CD)and(CWDI)were positively correlated.According to the CWDI value,the 42 maize varieties were divided into high drought resistance,medium drought resistance,and low drought resistance,among which included two high drought resistant varieties,and 13 medium drought resistant varieties,27 low drought resistant varieties.Variance analysis showed that the DI of yield of high resistance maize group was significantly higher than that of medium resistance and low resistance.In conclusion,the above six physiological indexes of maize leaves combined can be used as reference indexes for physiological identification of drought resistance of maize varieties at the grain filling stage.Liaodan 145 and Jinhua 150 have strong drought resistance among 42 tested varieties.

MaizeDrought resistanceIdentification indexGrey correlation analysisSystem clustering analysis

李春情、刘翔宇、闫鹏、周六千、卢霖、董志强、徐江

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中国农业科学院作物科学研究所/农业农村部作物生理生态重点实验室,100081,北京

新疆农业科学院吐鲁番农业科学研究所,838000,新疆吐鲁番

新疆大学生命科学与技术学院,830046,新疆乌鲁木齐

玉米 抗旱性 鉴定指标 灰色关联度分析 系统聚类分析

国家自然科学基金国家重点基础研究发展计划(973计划)课题中国农业科学院基本科研业务费专项院级统筹项目

315715892015CB150401Y2017PT25

2024

作物杂志
中国作物学会 中国农科院作物科学研究所

作物杂志

CSTPCD北大核心
影响因子:0.821
ISSN:1001-7283
年,卷(期):2024.(4)