Four national-approved wheat varieties(Luomai 163,Luomai 47,Luomai 49,and Luomai 36)were examined for the presence of key functional genes,such as photoperiod,grain weight,quality,spike sprouting,drought and disease resistance by using KASP marker detection.The results showed that,the four wheat varieties contained dwarfing gene Rht-D1b,allelic variants TaPRR73-A1 and TaPRR73-B1 related to plant height at maturity,photoperiod sensitive allele Ppd-A1b,photoperiod insensitive allele Ppd-Dla,high 1000-grain weight allele TaGS5-Ala,high grain number excellent variant Hap-7A-3,high quality subunit gene Ax1/Ax2* related to quality,high lipoxygenase activity allele Lox-B1a,anti-sprouting gene Rio Blanco type and TaSdr-B1a,and high lignin content excellent allele COMT-3Ba,all accounting for 100%.The utilization of hard genotype Pinb-D1b,high molecular weight glutenin subunit(5+10),stripe rust resistance gene Yr78 and leaf rust resistance gene Lr16 were higher in the four cultivars.High 1000-grain weight allele Hap-A,good allele variant of grain number per spike TaMoc-7A,grain hardness gene Pina-D1b/Null,Pinb-B2b,drought resistance gene 1-fehw3,powdery mildew resistance gene Pm21,and leaf rust resistance gene Lr46 and Lr48 were not detected.The findings of four significant characteristic functional genes of wheat varieties offered crucial reference information for the practical use of upcoming varieties.