Identification and Evaluation of Low Temperature Tolerance of 110 New Maize Materials at Germination Stage
In this study,12 low-temperature tolerance-related indexes,including the germination rate,germination index,germination potential,average germination time,seedling emergence rate,seedling emergence index and relative value of traits,were measured using 110 new maize materials as experimental materials adopting a combination model of field and indoor identification.Through ANOVA analysis,correlation analysis,principal component analysis and cluster analysis methods,we comprehensively evaluated the low-temperature tolerance of the new maize materials.The results showed that 110 new maize materials had abundant genetic variation in low-temperature germination ability,relative germination rate,relative germination index,and relative seedling emergence index could be used as important reference indicators for the identification of low-temperature tolerance during maize germination.Correlation analysis showed that the six relative traits were positively correlated with each other.The results showed that six single indicators were synthesized into two independent comprehensive indicators with a cumulative contribution rate of 94.20%by principal component analysis.At last,combined with cluster analysis,110 new maize materials were divided into six low-temperature tolerance grades,among which 27 had extremely strong low-temperature tolerance,22 had strong low-temperature tolerance,22 had moderately strong low-temperature tolerance,21 had weak low-temperature tolerance,16 had moderately weak low-temperature tolerance,and two had extremely weak low-temperature tolerance.
MaizeLow temperature toleranceGermination stagePrincipal component analysisCluster analysis