Using Fucaishu 18,a vegetable sweet potato variety,as the material,it was treated for 24,48,and 72 hours at 15 ℃ and 12 ℃,respectively,before applying a low-temperature stress treatment at 8 ℃/5 ℃.The physiological characteristics of antioxidant enzymes,osmotic mediators,membrane lipid peroxidation,and cold resistance were determined after stress 0,24,48,and 72 h after stress,as well as the restoration of room temperature 48,48,and 48 h again low-temperature stress.These data were used to examine the effects of various low-temperature acclimation times on the cold tolerance of sweet potatoes.The results showed that with the extension of the low-temperature stress time,the SOD and POD activities of the low-temperature acclimation leaves gradually increased.Although the contents of soluble protein and soluble sugar fluctuated,the change range was relatively smoother than CK.During the low-temperature stress period,the MDA content of each low-temperature acclimation treatment was gradually decreased,and CK gradually increased with the extension of the stressed time.It was observed that there was a significant interaction between low-temperature acclimation and low-temperature stress,and that the two factors jointly induced an improvement in cold resistance.The analysis showed that there was a significant interaction between low temperature exercise and low temperature stress,and cold tolerance jointly induced the improvement of cold tolerance under the piecewise interaction of the two factors.