Effects of Low Temperature Stress on Cold-Tolerance of Broomcorn Millet Varieties with Different Cold-Tolerance at Seedling Stage
Using the budding stage highly cold-tolerant resource Bameng Black millet and the highly cold-sensitive resource PAN21 as experimental materials,the effects of low temperature stress on growth indexes,osmotic adjustment and antioxidant capacity of different cold-tolerant millet varieties were compared through a pot experiment under artificial climate chamber conditions.The results showed that under low temperature stress,the dead seedling rate of Bameng Black millet was 8.9%,indicating that it was a strong cold-tolerant millet variety;the dead seedling rate of PAN21 was 80.3%,indicating that it was an extremely cold-sensitive millet variety,which was consistent with their cold-tolerance classification result in the budding stage.The effects of low temperature stress on the aboveground and underground parts of millet were significantly different;the contents of proline,soluble protein,MDA,and H2O2 in millet leaves under low temperature stress were all increased to varying degrees,and the chlorophyll content was reduced.Moreover,by increasing the activities of SOD and POD,the content of ROS was reduced,thereby alleviating the damage to the biological membrane caused by low temperature.The osmotic adjustment substances and the efficiency of antioxidant system are important physiological indexes for measuring the cold-tolerance of broomcorn millet.
Broomcorn milletCold-toleranceLow temperature stressGrowth indexPhysiological index