Diversity Analysis and Function Study of Culturable Endophytic Fungi in Oat Shoot
In order to investigate the distribution,community composition,and functional characteristics of culturable endophytic fungi leaves and stems of oats(Avena sativa L.),the endophytic fungi in leaves and stems of oats at the jointing stage in the northern foothills of Yinshan were isolated and purified by the tissue culture isolation method.Their diversity was identified and analyzed by using ITS,and the abilities of phosphate-solubilizing and potassium-solubilizing,secreting auxin(IAA)were measured.The results showed that a total of 74 endophytic fungi were isolated from the leaves and stems of oats,with the total colonization rate and the total isolation rate of 61.94％and 20.56％,respectively,they were identified as belonging to two phyla and 12 genera by molecular identification,among which Ascomycota was the dominant phylum,and Alternaria(isolation rate=5.56％,isolation frequency=27.03％)and Fusarium(isolation rate=8.06％,isolation frequency=39.19％)were the dominant species.Diversity analysis showed that the diversity of endophytic fungi isolated from stems was higher than those from leaves,with the diversity index and Simpson index being 3.51 and 0.78,respectively.Similarity analysis showed that the similarity coefficient of endophytic fungi in leaves and stems was 0.15.The functional analysis of the isolated endophytic fungi showed that 79.73％of the strains had phosphate-solubilizing,72.97％had potassium-solubilizing,and 40.54％could secrete IAA.According to the function of endophytic fungi,74 strains of endophytic fungi were divided into four categories,including 41,5,21 and 7 strains,respectively.Most of the optimal strains of the 4th category had the ability to dissolve phosphorus and potassium,and the ability to secrete IAA was the highest.There are abundant endophytic fungi species in the leaves and stems of oat,which would provide strain resources for the application of endophytic fungi.The functions of different endophytic fungi are quite distinct,and the strains with stronger functions may exert their potential growth-promoting characteristics in agricultural production,which is beneficial to the sustainable development of agriculture.
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