Investigation on Main Viral Diseases in Virus-Free Seed Potato in Ningxia
Potato is an important food and cash crop in Ningxia,one of the primary factors influencing potato productivity and quality is potato virus disease,and the most effective method of controlling potato virus disease is detoxifying seed potato.In order to investigate the virus-carrying situation of virus-free seed potato and to clarify the occurrence trend of seed potato virus diseases in Ningxia,a total of 1105 seed potato samples,including virus-free test-tube seedlings,breeder seed,pedigree seed,first-degree species and other types of potato were collected from major virus-free seed potato production counties(districts,farms)of Ningxia from 2019 to 2023,and then all the samples were detected to determine the carrying status of the six most serious viruses(PLRV,PVY,PVA,PVS,PVM and PVX)by DAS-ELISA technology.The results showed that six kinds of potato viruses were carried in all tested potato produced in Ningxia,and there was an obvious regional difference.The detection rate of PLRV was the highest among all the virus,up to 4.16%,followed by PVY(3.98%),PVA(3.80%),PVS(3.62%)and PVM(2.17%),the PVX was the lowest,only 1.63%.The presence of a single virus(11.12%)and multiple viruses(2.97%)existed simultaneously in seed potato,but overall virus carrying rate of seed potato showed a decreasing trend year by year.
Seed potatoVirus diseasesDAS-ELISAPrevention and control