首页|和枢消积方通过调节AsTP3正反馈AKT/GSK-3β/mTOR信号通路对人肝癌细胞增殖及凋亡的影响

和枢消积方通过调节AsTP3正反馈AKT/GSK-3β/mTOR信号通路对人肝癌细胞增殖及凋亡的影响

扫码查看
原文链接
  • NETL
  • NSTL
  • 万方数据
  • 维普
目的:探讨和枢消积方通过调节三氧化二砷反式激活蛋白3(AsTP3)对人肝癌细胞SMMC-7721 增殖、凋亡的作用机制.方法:体外培养SMMC-7721 肝癌细胞,采用CCK8 法筛选出和枢消积方最适浓度,联合CCK8 法与平板克隆形成实验检测细胞增殖,细胞划痕实验及Transwell小室实验检测细胞迁移、侵袭能力,流式细胞实验检测细胞凋亡,qRT-PCR检测细胞AsTP3 mRNA水平,Western Blot检测细胞中AsTP3、AKT、GSK-3β、mTOR蛋白相对表达量及磷酸化水平.结果:与对照组比较,和枢消积方能抑制SMMC-7721 细胞增殖、迁移、侵袭及克隆能力,促进SMMC-7721 细胞凋亡(P＜0.05);和枢消积方可下调SMMC-7721 细胞内AsTP3、P-AKT、P-GSK-3β、P-mTOR、Bcl2 的mRNA表达及蛋白水平并上调Bax蛋白的表达.结论:和枢消积方能抑制SMMC-7721 细胞增殖,促进其凋亡,其机制可能与下调AsTP3 表达从而抑制AKT/GSK-3β/mTOR信号通路有关.
Effects of Heshuxiaoji formula on proliferation and apoptosis of human hepatoma cell line by regulating AKT/GSK-3β/mTOR signaling pathway with AsTP3 positive feedback
Objective:To investigate the effect of Heshuxiaoji formula on the proliferation and apoptosis of human hepatoma cell SMMC-7221 by regulating the arsenic trioxide-activated protein 3(AsTP3).Methods:SMMC-7721 hepatoma cells were cultured in vitro and the optimal concentration of Heshuxiaoji formula was selected by using CCK 8.CCK8 and plate colony-forming assay were used to detect cell proliferation activity,cell migration by cell wound scratch assay,cell invasion by Transwell,and cell apoptosis by Flow cytometry.qRT-PCR and Western Blot were used to detect the expression of apoptosis-related genes such as AsTP3.Results:Compared with the control group,Heshuxiaoji formula could inhibit the proliferation,migration,invasion,and cloning of SMMC-7721 cells,and promote the apoptosis of SMMC-7721 cells.The Heshuxiaoji formulation may attenuate the messenger RNA and protein expression levels of AsTP 3,p-AKT,P-GSK-3β,P-mTOR,and Bcl2,while concurrently augmenting the protein expression of Bax within SMMC-7721 cells.Conclusion:Heshuxiaoji decoction can inhibit the proliferation of SMMC-7721 cell and promote their apoptosis,which may be related to the down-regulation of AsTP3 expression and the inhibition of AKT/GSK-3β/mTOR signaling pathway.

hepatocellular caroinomaHeshuxiaoji decoctionarsenic trioxide-actived protein3AKT/GSK-3β/mTOR signaling pathuay

李玲、汪静、朱晓宁、张玉蓉、尹玥

展开 >

西南医科大学附属中医医院肝胆病科(四川 泸州,646000)

肝癌 和枢消积方 三氧化二砷反式激活蛋白3 AKT/GSK-3β/mTOR信号通路

四川省科学技术厅重大科技专项泸州市人民政府-西南医科大学科技战略合作项目西南医科大学附属中医医院国家中医临床研究基地建设单位科研项目西南医科大学-西南医科大学附属中医医院联合项目西南医科大学-西南医科大学附属中医医院联合项目

2019YFS00182019LZXNYDZ12西南医大中医院[2020]33号2020XYLH-0372018XYLH-030

2024

10.3969/j.issn.1005-0264.2024.002.008

中西医结合肝病杂志
中国中西医结合学会,湖北中医学院

中西医结合肝病杂志

CSTPCD
影响因子：0.908
ISSN：1005-0264
年,卷(期)：2024.34(2)
  • 19