The effect of Heshu Xiaoji prescription on tumor suppression and tumor tissue VEGF and PDGF proteins in H22 tumor-bearing mice
Objective:To investigate the effects of Heshu Xiaoji prescription(HXP)on tumor suppression and expression of tumor angiogenesis-related factors in H22 tumor-bearing mice,to elucidate its potential mechanism,and to explore the effective anti-hepatocellular carcinoma therapeutic options combining Chinese and Western medicine.Methods:H22 tumor-bearing mice were randomly divided into blank group,model group,low,medium and high dose groups of HXP(1.37,2.73,5.46 g/kg),sorafenib group(0.03 g/kg),combined group(5.46 g/kg HXP combined with0.03 g/kg sorafenib),and the intervention was carried out for 14 days.After the experiment,tumor volume and tumor suppression rate were calculated,aspartate aminotransferase(AST)and alanine aminotransferase(ALT)liver function indexes were measured,tumor cell structure and morphology were observed by Hematoxylin-eosin staining(HE)and vascular endothelial growth factor(VEGF)and platelet-derived growth factor(PDGF)protein expression levels were measured by immunohistochemistry and Western blotting in the tumor tissues of each group.Results:There was no significant effect on body weight change in mice,and HXP improved the mental and activity status and diarrhea symptoms caused by sorafenib in H22 tumor-bearing mice.Compared with the control group,all treatment groups inhibited the volume and mass of subcutaneous tumors in tumor-bearing mice,among which the relative tumor proliferation rate and tumor inhibition rate in the combined group were31.3%and74.8%,respectively,with the most obvious effect(P<0.05).HXP down-regulated AST and ALT expression levels and improved liver function in mice(P<0.01).HE staining showed that the internal structure of tumor tissues was disturbed in each treatment group,which was positively correlated with the dose of HXP,and the most significant in the combined group.IHC and Western Blotting experiments revealed that the protein expression of VEGF and PDGF were decreased in each treatment group compared with the model group(P<0.01);the combined group inhibited VEGF and PDGF more significantly than the single sorafenib or herbal medicine group.Conclusion:HXP can down-regulate tumorigenesis-related factors VEGF and PDGF protein expression,and has a tumor-suppressive effect on H22 bearing mice,which is most significantly treated with a combination of Chinese and Western medicine in combination with sorafenib.
NETL
NSTL
万方数据
维普
