Experimental study on the proliferation and migration of drug containing serum of bushenhuatan formulae on HepG2 hepatoma cells
Objective:To study the effects of Bushenhuatan Formulae(BHF)on organ index of mice and BHF-containing serum on the proliferation and migration of HepG2 hepatocellular carcinoma cells.Methods:Mice were randomly divided into control group,BHF high-dose group,BHF medium-dose group,BHF low-dose group,and after gavage for 7 days,blood was collected to prepare drug-containing serum,and samples were taken to determine the organ index of mice in each group after execution.HepG2 hepatocellular carcinoma cells were cultured in vitro,and the blank group,control group containing drug-containing serum,BHF high-dose group containing drug-containing serum,BHF medium-dose group containing drug-containing serum,BHF low-dose group containing drug-containing serum were set up with three concentrations in each group(20％,10％,and 5％),and 3 replicate wells were set up in each group.The level of cell proliferation in each group was detected by CCK8 method after 24 h,48 h and 72 h of culture after adding the corresponding drugs in each group.Morphological changes of cells in each group were observed microscopically after 24 h of culture using transwell cells.The subsequent experiments were carried out after screening the optimal effector-containing serum concentration.The level of HepG2 cell migration in each group was detected using cell scratch assay.Results:Cell proliferation showed that at 24 h,the cell proliferation inhibition rate was significantly lower in the BHF low-dose group than in the control group(P＜0.05),the BHF high-dose group(P＜0.01),and the BHF medium-dose group(P＜0.01).At 48 h,the cell proliferation inhibition rate in the BHF medium-dose group was significantly higher when compared with that in the control group(P＜0.05),and significantly higher than that in the BHF low-dose group(P＜0.01).At 72 h,the cell proliferation inhibition rate was significantly higher in all BHF dose groups compared with the control group(P＜0.01).And the cell inhibition rate of BHF high dose group was significantly higher than that of BHF middle dose group(P＜0.01).The cell inhibition rate of BHF middle dose group was significantly higher than that of BHF low dose group(P＜0.05)Cell culture showed a decrease in the number of cells in each BHF dose group.Cell scratch showed that 72 h scratch migration rate,was significantly lower in BHF high dose group compared to blank group(P＜0.05).Conclusion:BHF-containing serum inhibited the proliferation and migration of HepG2 hepatocellular carcinoma cells,and the BHF high-dose group showed the best performance.
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万方数据
维普
