青蒿琥酯下调lncRNA HOTAIR表达抑制肝癌细胞增殖及迁移作用
Artesunate down-regulates lncRNA HOTAIR expression and inhibits proliferation and migration of hepatocellular carcinoma cells
楼衷晗 1包剑锋1
作者信息
- 1. 浙江大学医学院附属杭州市西溪医院肝病三科(浙江 杭州,310000)
- 折叠
摘要
目的:探讨青蒿琥酯通过抑制lncRNA HOTAIR表达对人肝癌细胞增殖、迁移能力影响及其作用机制.方法:分别采用MTT法、Transwell小室法检测不同浓度青蒿琥酯对人肝癌细胞Huh7、HepG2 增殖及迁移能力的影响;采用qRT-PCR法检测青蒿琥酯对Huh7 细胞HOTAIR表达影响;使用HOTAIR过表达质粒转染Huh7 细胞,qRT-PCR验证其转染效率;不同浓度青蒿琥酯处理正常Huh7 细胞和Huh7+HOTAIR-OE细胞,MTT法、Transell小室法检测各组细胞增殖、迁移能力变化,验证HOTAIR过表达对青蒿琥酯抑癌作用影响,进一步了解青蒿琥酯通过调控HOTAIR实现对肝癌细胞的抑制作用.结果:青蒿琥酯可抑制人肝癌Huh7、HepG2细胞增殖及迁移能力,且呈剂量依赖性(P＜0.05);青蒿琥酯可抑制Huh7 细胞HOTAIR表达;HOTAIR过表达后减弱了青蒿琥酯抑制Huh7 细胞的增殖及迁移能力(P＜0.05).结论:青蒿琥酯可能是通过或者部分通过下调lncRNA HOTAIR表达,从而达到抑制肝癌细胞增殖和迁移能力.
Abstract
Objective:To investigate the effect and mechanism of Artesunate on the proliferation and migration of human hepatocellular carcinoma cells by inhibiting the expression of lncRNA HOTAIR.Methods:The effects of different concentrations of artesunate on the proliferation and migration of Huh7 and HepG2 cells were detected by MTT assay and Transwell chamber method.The effect of ART on HOTAIR expression in Huh7 cells was detected by qRT-PCR method.Huh7 cells were transfected with HOTAIR overexpression plasmid,and the transfection efficiency was verified by qRT-PCR.Normal Huh7 cells and Huh7+HOTAIR-OE cells were treated with different concentrations of ART,and then MTT assay and Transell chamber method were used to detect the changes of proliferation and migration ability of ART.To verify the effect of HOTAIR overexpression on ART on hepatoma,and to further understand whether ART can inhibit hepatoma cells by regulating HOTAIR.Results:ART inhibited the proliferation and migration of human hepatoma Huh7 and HepG2 cells in a dose-dependent manner.ART could inhibit the expression of HOTAIR in Huh7 cells.Overexpression of HOTAIR attenuated the inhibitory effect of ART on proliferation and migration of Huh7 cells.Conclusion:ART may inhibit the proliferation and migration of hepatocellular carcinoma cells through or partly down-regulating the expression of lncRNA HOTAIR.
关键词
青蒿琥酯/长链非编码RNA/HOTAIR/肝癌细胞/增殖/迁移Key words
artesunate/long noncoding RNA/HOTAIR/hepatoma cell/proliferation/migration引用本文复制引用
基金项目
浙江省自然基金项目(LSY19H030002)
杭州市卫生局项目(2018A48)
出版年
2024
国家科技期刊平台
NSTL
万方数据