Immune role and prognostic value of zinc finger protein 605 in hepatoce-llular carcinoma
Objective:To investigate the immune function and prognostic value of zinc finger protein 605(ZNF605)in hepatocellular carcinoma.Methods:The Cancer Genome Atlas Program(TCGA)and Gene Expression Omnibus(GEO)were used to analyze the expression of ZNF605 in liver cancer,and the correlation between its expression level and clinical pathological characteristics of liver cancer patients was analyzed.The UALCAN database explored the methylation expression levels of ZNF605 in liver cancer and adjacent tissues.ROC analyzed the diagnostic value of ZNF605 in liver cancer and plotted a nomogram to predict the 1-year,3-year,and 5-year overall survival probabilities of liver cancer patients.The prognostic value of ZNF605 in liver cancer was analyzed using univariate and multivariate Cox regression analysis,Gene enrichment analysis(GSEA)was conducted on the high and low expression of ZNF605.The correlation between the expression level of ZNF605 and the immune cells in the tumor microenvironment was analyzed by R package GSVA.Results:Our research showed that ZNF605 was abnormally expressed in various cancers and was highly expressed in liver cancer,with low methylation levels in liver cancer.High expression of ZNF605 was associated with patient T stage,clinical stage,histological grade,overall survival,and alpha fetoprotein.Univariate and multivariate Cox regression analysis showed that ZNF605 expression and T stage were independent prognostic risk factors for liver cancer.GSEA analysis showed that high expression of ZNF605 was mainly enriched in the cell cycle.Immune cell infiltration analysis suggested that the expression level of ZNF605 in liver cancer was significantly positively correlated with Th2 cells and T helper cells.On the contrary,the expression of ZNF605 was negatively correlated with dendritic cells,Th17 cells,cytotoxic cells,neutrophils,plasma cell like dendritic cells,B cells,Tgd cells and mast cell.Conclusion:Bioinformatics analysis indicate that the highly expressed ZNF605 in liver cancer may be a biomarker for the diagnosis and prognosis of liver cancer,as well as a potential therapeutic target.
Hepatocellular carcinomazinc finger protein 605bioinformaticsimmune infiltrationdiagnosisprognosis
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