Clinical application of contrast-enhanced ultrasound combined with serum miR-125b and miR-140 in the diagnosis of benign and malignant liver occupying lesions
Objective:To investigate the efficacy and value of miR-125b and miR-140 combined with contrast-enhanced ultrasound in the diagnosis of benign and malignant liver occupying lesions.Methods:A total of 125 patients with liver space occupying lesions admitted to our hospital from June 2022 to June 2023 was included and retrospectively analyze,according to the pathological biopsy results,they were grouped into a benign group(n=65)and a malignant group(n=60),and healthy individuals were collected as the control group(n=56),qRT-PCR experiment was applied to detect the relative expression levels of miR-125b and miR-140 in serum,and contrast-enhanced ultrasound examination was performed on patients.The basic clinical data of patients were collected and analyzed.The receiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of serum miR-125b,miR-140 combined with contrast-enhanced ultrasound in patients with benign and malignant liver occupying lesions.Results:Compared with the control group,the serum levels of miR-125b and miR-140 in patients in the liver space occupying lesions group were obviously reduced(P＜0.05),and the serum levels of miR-125b and miR-140 in malignant group were also obviously lower than benign group(P＜0.05).Ultrasonographic results diagnosed 68 patients with benign lesions and 57 patients with malignant lesions,and further analysis of the ultrasonographic features revealed that the proportion of patients in the malignant group with early enhancement,hyper-enhancement,and fast fading was significantly higher than that in the benign group(P＜0.05).Clinical basic data analysis found that the Alanine aminotransferase(ALT),Aspartate aminotransferase(AST),and Alkaline phosphatase(ALP)levels in the malignant group were obviously higher than benign group(P＜0.05),and there was an obvious statistical difference in tumor diameter and pathological type between the two groups(P＜0.05),there was no statistical difference in age and gender between the two groups(P＞0.05).Drawing ROC curves revealed that the AUC of serum miR-125b,miR-140,and their combined diagnosis was 0.768,0.841,and 0.903,respectively,the specificity and predictive accuracy of serum miR-125b,miR-140,and their combined diagnosis with contrast-enhanced ultrasound was higher than those of miR-125b,miR-140,and contrast-enhanced ultrasound alone.Conclusion:Serum miR-125b and miR-140 combined with ultrasonography have high clinical value in the diagnosis of benign and malignant liver occupying lesions.
liver space occupying lesionscontrast-enhanced ultrasoundmiR-125bmiR-140
万方数据
维普
