Evaluation of the safety and efficacy of different fecal bacteria transplantation based on intestinal flora of healthy people and nonalcoholic fatty liver disease patients
Objective:This study explores the potential causal relationship between dysregulated gut microbiota and the progression of non-alcoholic fatty liver disease (NAFLD).Using fecal microbiota transplantation (FMT)via oral gavage or enema,gut microbiota from NAFLD patients and healthy individuals were introduced into Sprague-Dawley rats and NAFLD model rats.Furthermore,the study evaluates the therapeutic effects of FMT from healthy donors on NAFLD model rats.Methods:Thirty-five male SD rats were randomly divided into blank group (group N),healthy human bacterial solution+gavage+SD rat group (WN group),NAFLD patient bacterial solution+gavage+SD rat group (WH group),healthy human bacterial solution+enema+SD rat group (CN group),NAFLD patient bacterial solution+enema+SD rat group(CH group).NAFLD rat model group (group M),NAFLD rat model+healthy human bacterial solution group (group Z),5 mice in each group.After the experiment,The liver histomatology,liver enzymology index (ALT,AST),lidexes levels (TC,TG,GLU)and intestinal flora characteristics of all groups were compared.Results:Compared with group N,a small amount of inflammatory cell infiltration and balloon-like transformation were observed locally in the liver tissue of rats in WN,WH,CN and CH groups,AST,ALT,TC and TG levels were increased,and intestinal flora structure was significantly different among groups.Compared with WN and CN,pathological changes such as inflammatory response and ballooning were more obvious in WH and CH groups,NAS scores were increased,and ALT,AST,TC and TG levels were increased.There was a significant difference in intestinal flora composition between WN and WH groups,while there was no significant difference between CN and CH groups.Compared with WN and WH groups,lobular inflammation,balloon-like transformation and other overall pathological changes were more obvious in CN and CH groups,NAS scores were increased,ALT and AST levels were increased,and intestinal flora structure was significantly different between groups.However,TC level in CN group was decreased compared with WN group,and TC and TG levels in CH group were lower than those in WH group.Compared with group N,the liver tissues of rats in groups M and Z showed obvious pathological manifestations such as steatosis,lobular inflammation and balloon-like transformation,and the levels of NAS score,ALT,AST,TC and TG were significantly increased (P<0.05 ),and the levels of NAS score,ALT,AST,TC and TG were significantly higher than those in group Z than group M,with significant differences in microflora structure between groups.Conclusion:Transplantable human intestinal flora can induce liver pathological changes,abnormal liver enzymology and lipid metabolism disorders by affecting intestinal flora structure in SD rats.Compared with healthy people,fecal bacteria in NAFLD patients can aggravate liver inflammation and lipid metabolism disorder in SD rats;Pedestrian intestinal microbial transplantation by enema can easily induce liver inflammation in SD rats,while gavage intervention may be more likely to cause lipid metabolism disorders.Fecal bacteria solution of healthy human can alleviate the inflammatory response of NAFLD model rats,improve the disorder of lipid metabolism,and delay the progression of NAFLD disease.
万方数据
维普
