Chinese Herbs for Treatment of Heart Failure Based on the Perspective of Iron Death
Objective:To screen traditional Chinese medicine for the treatment of heart failure(HF)based on the perspective of iron death.Methods:HF related eligible datasets in GEO database were retrieved,limma differential analysis were carry out to obtain HF differential expression targets using R software,iron death related targets were collected on FerrDb platform,protein-protein interaction network was constructed after intersection(PPI)of iron death targets and HF differential expression targets,and topological properties of the network were analyzed.Gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were performed for common targets using R software.The HERB database was used to search the compounds and traditional Chinese medicine corresponding to the common target,and the small molecule compounds and their corresponding targets were used to carry out molecular pairs.Results:Two datasets were screened from GEO database,there were 14 093 targets obtained through limma differential analysis,214 targets related to iron death were obtained through collection,176 targets were obtained through intersection of iron death and HF differential expression targets,and key targets TP53,JUN,UBC,MAPK14,MAPK3,STAT3,EGFR,etc.were obtained through PPI network construction and topological property analysis of common targets of iron death and HF differential expression.The enrichment analysis of GO and KEGG showed that HF was related to cell response to chemical stress,oxidative stress,secondary lysosome,melanosome,autophage,outer membrane of mitochondria,nucleus of basement membrane,oxidoreductase activity,iron binding,lipid and atherosclerotic,FoXO signal pathway,etc.from the perspective of iron death.The results of searching HERB database showed that nearly 80 kinds of compounds of natural drugs,such as berberine,withaferin A,sanguinarine,and more than 100 kinds of traditional Chinese medicines,such as cortex dictamni,scutellaria barbata,atractylodes,andrographis paniculata,and fangfeng could be used to treat HF by acting on key targets.Molecular docking results showed that JUN and berberine,MAPK14 and withaferin A,MAPK3 and sanguinarine could well combine.Conclusion:Through bioinformatics research,it is found that with berberine,withaferin A,sanguinarine and other compounds,as well as some traditional Chinese medicines such as Coptis,Radix Stephaniae,Cortex Phellodendri,Rhizoma Corydalis,Radix Achyranthes,Fructus Evodiae,Radix Astragali,Radix Salviae Miltiorrhizae can act on TP53,MAPK14,MAPK3,JUN,STAT3,and other targets to treat HF.
NETL
NSTL
万方数据
维普
