Protective Effect and Mechanism of Rhein on H9c2 Cardiomyocytes Injury Induced by High Glucose
Objective:To observe the protective effect of rhein(RH)on H9c2 cardiomyocytes injury induced by high glucose and its possible mechanism.Methods:H9c2 cells were divided into normal glucose group(NG group,glucose concentration was 5.5 mmol/L),high glucose group(HG group,glucose concentration was 35 mmol/L),high glucose+rhein group(HG+RH 5 group,HG+RH 10 group,HG+RH 30 group).The concentration of rhein was 5μmol/L,10μmol/L,and 30μmol/L,respectively.Cell viability was detected by cell counting kit-8(CCK-8).Protein expression of silencing information regulator 1(Sirt1),peroxisome proliferator-activated receptor-γ-coactivator-1α(PGC-1α)and mitochondrial transcription factor A(TFAM)were detected by Western Blot.H9c2 cells were divided into normal glucose group(NG group),high glucose group(HG group),high glucose+rhein group(HG+RH10 group,rhein 10μmol/L),high glucose+rhein +EX527 group(HG+RH10+EX527 group,EX527 10μmol/L).Real-time fluorescence quantitative polymerase chain reaction(PCR)was used to detect mRNA levels of Sirt1,PGC-1α,TFAM,nuclear respiratory factor 1(NRF-1)and uncoupling protein 2(UCP2).The expressions of Sirt1,PGC-1αand TFAM were detected by Western Blot.Results:Compared with NG group,the cell viability of HG group decreased(P<0.05).The cell viability of different concentrations of rhein group increased to a certain extent,but the difference was not statistically significant(P>0.05).Compared with NG group,the protein levels of Sirt1,PGC-1αand TFAM in HG group significantly decreased(P<0.05).Compared with HG group,the protein levels of Sirt1,PGC-1αand TFAM in RH 10μmol/L group increased(P<0.05).Compared with NG group,mRNA levels of Sirt1,PGC-1α,TFAM,NRF-1 and UCP2 in HG group decreased(P<0.05).Compared with HG group,the above mRNA levels in HG+RH10 group increased,and EX527 inhibited the increase of rhein(P<0.05).Conclusion:Rhein showed some aprotective effect on cardiomyocytes injury induced by high glucose,which might be achieved by activating of Sirt1/PGC-1α pathway
diabetic cardiomyopathyrheincardiomyocytes injuryprotective effectSilencing information regulator 1,Sirt1,peroxisome proliferator activator receptor γ coactivator 1α,PGC-1αexperimental study
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