Mechanism of the "Same Treatment of Different Diseases" of Salvia Miltiorrhiza for Treating Coronary Heart Disease and Stroke Based on Network Pharmacology and Molecular Docking
Objective:To explore the heteropathy mechanism and key therapeutic targets of the"same treatment for different diseases"of salvia miltiorrhiza for the treatment of coronary heart disease(CHD)and stroke by network pharmacology and molecular docking.Methods:Potential targets for the treatment of coronary heart disease and stroke were screened through traditional Chinese medicine(TCM)System Pharmacology Database and Analysis Platform(TCMSP),online human Mendelian Genetic database(OMIM),DrugBank,and other databases.Target protein-protein interaction(PPI),Gene ontology(GO)and Kyoto Genome Encyclopedia(KEGG)pathway analysis via the Metascape online platform.The active ingredient-key target-core pathway network was constructed using Cytoscape 3.9.1 to screen out the core components.Molecular docking was used to simulate the degree of binding between core components and key targets.Results:Fifty-two potential targets of salvia miltiorrhea for the treatment of coronary heart disease and stroke were selected,mainly concentrated in the pathways of advanced glycation end products(AGE)/receptor for advanced glycation end products(RAGE),platelet activation,lipid and atherosclerosis,fluid shear stress and atherosclerosis.The core pharmacodynamic components stably bound to phosphatidylinositol-3-kinase(PI3K),protein kinase B(AKT),nitric oxide synthase(NOS3,eNOS),B lymphocytoma-associated protein-2(Bcl-2),cyclooxygenase 2(PTGS2),integrin subunit α2b(ITGA2B),matrix metalloproteinase 9(MMP9),and other targets.Conclusion:The"same treatment for different diseases"of salvia miltiorrhoea can improve atherosclerosis in coronary heart disease and stroke mainly through anti-platelet activation and aggregation,protection of cardiovascular and cerebrovascular cells,stabilization of plaque,and reduction of inflammation,with potential target competition and synergistic effects.
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