Effect of Sijunzi Decoction on Ischemic Heart Failure:an Analysis Based on Network Pharmacology,Microarray Data Analysis,and Molecular Docking
Objective:To investigate the mechanism of Sijunzi Decoction(SJZD)in the treatment of ischemic heart failure through network pharmacology,microarray data analysis,and molecular docking.Methods:R software was used to analyze the microarray data of ischemic heart failure(HF)from the GEO database.The active components of 4 herbs in SJZD and targets corresponding to each component were scanned out from Traditional Chinese Medicines Systems Pharmacology Platform(TCMSP).The matching target of active components between SJZD and heart failure was obtained through network pharmacology.The protein-protein interaction(PPI)network was constructed by the Cytoscape software(v 3.2.1)and the STRING database.Gene ontology(GO)enrichment and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analyses were executed by clusterProfiler(v 3.8)and DOSE(v 3.6)package on R platform.Finally,AutoDockTool and PyMOL software were used for preprocessing and molecular docking.Results:A total of 371 differentially expressed genes(DEGs)related to heart failure were obtained through the analysis of microarray data.A total of 136 active ingredients and 4 134 targets of SJZD were picked out.Nine matching target genes and 5 relative active compounds were selected for subsequent analyses and the"SJZD-HF"PPI network was constructed.Through GO biological process and KEGG enrichment analysis,important biological processes such as cell response to acidic chemicals,transport of organic hydroxyl compounds,axon regeneration,neuronal projection regeneration,endoplasmic reticulum lumen,collagen trimer formation,protein binding and focal adhesion pathway were obtained.The results of molecular docking showed that naringin exhibited strong binding affinity with apolipoprotein B(APOB)and complement C1q binding protein(C1QB).Conclusion:The mechanism of SJZD for treating ischemic heart failure may be the direct or indirect action of multi-target and multi-pathway,which provides biological basis for the subsequent study of pharmacological mechanism of SJZD.
ischemic heart failureSijunzi Decoctionnetwork pharmacologymolecular dockingmicroarray data analysismechanism
万方数据
维普
