Study on Buyiqiangxin Tablet for the Treatment of Chronic Heart Failure Based on Network Pharmacology
Objective:To determine the effective components of Buyiqiangxin Tablet and its target for the treatment of chronic heart failure,and its potential mechanism.Methods:The effective components and predictive targets of Buyiqiangxin Tablet were obtained through the database of Traditional Chinese Medicine System Pharmacological Analysis Platform(TCMSP).The disease targets of chronic heart failure were screened by Disease Database,and the common targets of drugs and diseases were obtained by online tool Draw Venn Diagram.The common targets were uploaded to STRING database for protein-protein interaction(PPI)network analysis.Then,the active ingredient-target network diagram and the visual analysis of PPI network were constructed by Cytoscape 3.9.0 software.Then,gene ontology(GO)enrichment analysis and Kyoto Encyclopedia of Gene and Genome(KEGG)enrichment analysis were carried out through DAVID Database.Finally,Autodock Vina was used for molecular docking.Results:A total of 115 chemically active components,247 drug targets,2685 disease targets,and 153 common targets related to drugs and diseases were obtained.The core targets such as protein kinase 1(AKT1),interleukin-6(IL-6),vascular endothelial growth factor A(VEGFA),TP53,interleukin-1β(IL-1β),Caspase-3(CASP3),epidermal growth factor receptor(EGFR),JUN,matrix metalloproteinase 9(MMP-9),and prostaglandinendoperoxide synthase-2(PTGS2)were determined by PPI network analysis.The results of enrichment analysis showed that the potential core drug components showed pharmacological effects on chronic heart failure by inhibiting inflammatory reaction,regulating biological processes such as angiogenesis and apoptosis,advanced glycation endproducts(AGE)-receptor for AGE(RAGE)signal pathway of diabetic complications,cancer pathway,fluid shear stress and atherosclerosis,tumor necrosis factor(TNF)signal pathway,IL-17 signal pathway and so on.The results of molecular docking indicated that the key targets in the regulatory network exhibited high binding activity with related active components.Conclusion:This study screens the main active compounds,key targets,and main pathways for the treatment of chronic heart failure,and reveals its potential mechanism of Buyiqiangxin Tablet,so as to provide theoretical basis and reference value for further research.
万方数据
维普
