首页|天香丹抑制P2X7/NLRP3表达改善动脉粥样硬化的作用机制研究

天香丹抑制P2X7/NLRP3表达改善动脉粥样硬化的作用机制研究

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目的:探讨天香丹对动脉粥样硬化小鼠嘌呤能配体门控离子通道 7(P2X7)、核苷酸结合寡聚化结构域样受体蛋白 3(NLRP3)的影响。方法:选取 8周龄无特定病原体(SPF)级雄性载脂蛋白 E基因敲除(ApoE-/-)小鼠 30 只，予以高脂饲料喂养 12 周，造模成功后随机分为模型组、阿托伐他汀组、天香丹组，每组 10只;另选取 C57BL/6J小鼠 10只予普通饲料喂养，设为空白对照组。酶联免疫吸附法(ELISA)检测血清白细胞介素(IL)-18、IL-1β、腺嘌呤核苷三磷酸(ATP)的水平;免疫组化检测组织中 P2X7、NLRP3 的表达。结果:ELISA结果显示:与空白对照组相比，模型组 IL-18、IL-1β、ATP水平升高(P<0。05);与模型组相比，阿托伐他汀组、天香丹组IL-18、IL-1β、ATP水平降低(P<0。05)。免疫组化结果显示:与空白对照组相比，模型组 P2X7、NLRP3 表达均明显升高(P<0。05);与模型组相比，阿托伐他汀组、天香丹组 P2X7、NLRP3表达均明显降低(P<0。05)。结论:天香丹改善动脉粥样硬化与调节钾稳态抑制炎症小体的激活有关。
The Effect of Tianxiangdan on Atherosclerosis by Inhibiting P2X7/NLRP3 Expression
Objective:To investigate the effect of Tianxiangdan on purinergic ligand-gated ion channel 7(P2X7)and nucleotide-binding oligomerization domain-like receptor protein 3(NLRP3)in atherosclerotic mice.Methods:Thirty 8-week-old SPF male ApoE-/-mice were selected and fed with high-fat diet for 12 weeks.After successful modeling,they were randomly divided into model group,statin group,and Tianxiangdan group,with 10 mice in each group.Ten C57BL/6J mice were fed with normal diet as blank control group.The levels of interleukin(IL)-18,IL-1β,and adenosine triphosphate(ATP)in serum were detected by enzyme-linked immunosorbent assay(ELISA).The expression of P2X7 and NLRP3 in tissues was detected by immunohistochemistry.Results:ELISA results displayed that compared with the blank group,the levels of IL-18,IL-1β,and ATP in model group increased(P<0.05).Compared with the model group,the levels of IL-18,IL-1β,and ATP in statin group and Tianxiangdan group decreased(P<0.05).The results of immunohistochemistry showed that the expression of P2X7 and NLRP3 in model group was significantly higher than that in blank group(P<0.05).The expression of P2X7 and NLRP3 in the statin group and Tianxiangdan group decreased compared with the model group(P<0.05).Conclusion:The improvement of atherosclerosis by Tianxiangdan is related to regulating potassium homeostasis and inhibiting the activation of inflammasome.

atherosclerosisTianxiangdannucleotide-binding oligomerization domain-like receptor protein 3,NLRP3purinergic ligand-gated ion channel 7,P2X7adenosine triphosphateexperimental study

白银雪、张夏夏、吴丹丹、任珊、安冬青

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新疆医科大学,新疆名医名方与特色方剂学重点实验室(乌鲁木齐 830054)

动脉粥样硬化 天香丹 核苷酸结合寡聚化结构域样受体蛋白3 嘌呤能配体门控离子通道7 腺嘌呤核苷三磷酸 实验研究

国家自然科学基金新疆维吾尔自治区重点实验室项目新疆维吾尔自治区科技重大专项

820608612020D040062022A03019

2024

10.12102/j.issn.1672-1349.2024.07.010

中西医结合心脑血管病杂志
中国中西医结合学会　山西医科大学第一医院

中西医结合心脑血管病杂志

CSTPCD
影响因子：1.463
ISSN：1672-1349
年,卷(期)：2024.22(7)
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