The Mechanism of Shengmai Decoction(Dangshen Formula)for Treating Chronic Heart Failure Based on Network Pharmacology and Bioinformatics
Objective:To explore the active ingredients,targets and molecular pathways of Shengmai Decoction(Dangshen Formula)for the treatment of chronic heart failure(CHF)using network pharmacology and bioinformatics,and to clarify the molecular mechanism of Shengmai Decoction(Dangshen Formula)in the treatment of CHF.Methods:Potential targets of active ingredients and compounds of Shengmai Decoction(Dangshen Formula)were obtained by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP)and Batmatic-TCM platform.GeneCards,therapeutic target database(TTD),DrugBank and DisGeNET databases were searched to screen CHF-related targets.The protein interaction(PPI)network diagram was constructed by STRING database,and Cytoscape software was used to obtain the key compounds and core targets of CHF acted by Shengmai Decoction(Dangshen Formula).The R language software package was used to analyze the biological process of gene ontology(GO)and the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway.The CHF gene expression chip was downloaded from the integrated gene expression database(GEO)and the differential expression of core target genes was verified after processing with the R language software package.The molecular docking of the active ingredient and the core target was verified again by the Autodock software,and the results were visualized and displayed.Results:A total of 35 active chemical components and 120 potential targets were screened,and the active components such as spinach sterol,11-hydroxylanguinarianserine,α-spinach sterol,diisooctyl phthalate,soybean xanthin,and deoxynivalenol base,the core targets such as serine/threonine kinase(AKT1),non-receptor tyrosine kinase(SRC),and epidermal growth factor receptor(EGFR),and the key pathways including oxidative stress,tyrosine phosphorylation modification,phosphatidylinositol-3 kinase/protein kinase B(PI3K/AKT)were obtained.The results of GEO microarray validation showed that the core targets were differentially expressed between the healthy and diseased groups.The results of molecular docking showed that the key active ingredients of Shengmai Decoction(Dangshen Formula)were well combined with the core targets.Conclusion:Shengmai Decoction(Dangshen Formula)might improve chronic heart failure through AKT1,SRC,EGFR,heat shock proteins(HSP90AA1),prostaglandin endoperoxide synthase 2(PTGS2),matrix metalloproteinases 9(MMP-9)and other multi-targets and PI3K/AKT signaling pathway,and improve cardiac remodeling,inhibit cell apoptosis,regulate estrogen,anti-lipid and atherosclerosis.remodeling,inhibition of apoptosis,regulation of estrogen,anti-lipid and atherosclerotic.
NETL
NSTL
万方数据
维普
