Transcriptomic Mechanism of Lingbao Huxin Pill for the Treatment of Atherosclerosis
Objective:To study the therapeutic effect and potential mechanism of Lingbao Huxin Pill in improving atherosclerosis.Methods:C57BL/6 mice were fed with normal diet,and apolipoprotein E gene knockout(ApoE-/-)mice were fed with high-fat diet to induce the atherosclerosis model.After 12 weeks,the mice were randomly divided into model group,Lingbao Huxin Pill low-dose group,Lingbao Huxin Pill medium-dose group,Lingbao Huxin Pill high-dose group and statin group.After 8 weeks of administration,the levels of serum total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C)and high density lipoprotein cholesterol(HDL-C)were detected.The pathological changes of aortic root were detected by oil red O staining,Masson staining and hematoxylin-eosin(HE)staining.The mechanism of alleviating atherosclerosis of Lingbao Huxin Pill was analyzed by transcriptomic sequencing.Result:Compared with model group,the levels of plaque,TC,TG and LDL-C in the Lingbao Huxin Pill groups significantly decreased,the collagen area of plaque was significantly cells increased,and inflammatory infiltration was decreased.Transcriptomic sequencing results showed that compared with the model group,733 differential genes were screened out in the Lingbao Huxin Pill groups and the pathways enriched included fatty acid biosynthesis,collagen formation,and inflammation signaling pathways.Conclusion:Lingbao Huxin Pill could significantly improve atherosclerosis,and the mechanism might involve the key pathways of lipid metabolism and inflammatory response.
atherosclerosisLingbao Huxin Pilltranscriptomicslipidsexperimental study
NETL
NSTL
万方数据
维普
