Effects of Acupuncture"Jieyu Formula"on Hypothalamic-pituitary-adrenal Axis and BDNF/TrkB/CREB Pathway in Rats with Chronic and Unpredictable Mild Stress-induced Depression
Objective:To observe the effects of"Jiyu Formula"on the hypothalamic-pituitary-adrenal(HPA)axis and brain-derived neurotrophic factor(BDNF)/tyrosine kinase B receptor(TrkB)/cyclic adenosine phosphate reaction element binding protein(CREB)signaling pathway on chronic and unpredictable mild stress(CUMS)depressed rats.Methods:Forty specific pathogen free(SPF)grade SD male rats were randomly divided into blank group(10 rats),model group(10 rats),western medicine group(10 rats),and acupuncture group(10 rats).Except for the blank group,the other three groups were continuously used to construct a depression rat model by CUMS for 28 days.After successful modeling,the western medicine group was gavaged Paxil hydrochloride suspension once a day for 14 days.In the acupuncture group,Baihui,Taichong and Shenmen were acupunctured once a day for 20 minutes each time for 14 days.The pathological changes of hippocampus were detected by hematoxylin-eosin(HE)staining,and the levels of serum adrenocorticotropin releasing hormone(CRH),adrenocorticotropin(ACTH)and cortisol(CORT)were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of BDNF and TrkB in hippocampus were detected by immunohistochemistry(IHC),and the expressions of BDNF,TrkB and CREB protein and mRNA in hippocampus were detected by Western Blot and real-time fluorescence quantification and polymerase chain reaction(PCR).Results:Compared with blank group,the contents of serum CRH,ACTH and CORT in the model group significantly increased(P<0.01),the hippocampal pathological injury was serious,the average optical density of BDNF and TrkB in hippocampus significantly decreased(P<0.01).The protein and mRNA levels of BDNF,TrkB,and CREB were significantly decreased(P<0.05 or P<0.01).Compared with model group,the contents of serum CRH,ACTH and CORT in the acupuncture group significantly decreased(P<0.05),the hippocampal pathological injury was significantly alleviated,the average optical density of BDNF and TrkB significantly increased(P<0.05)The protein and mRNA levels of BDNF,TrkB,and CREB were significantly increased(P<0.05).Conclusion:Acupuncture"Jieyu Formula"could improve depression-like behavior induced by CUMS in rats by regulating HPA axis and BDNF/TrkB/CREB signaling pathway.
depressionchronic unpredictable mild stressacupoint"Jieyu Formula"hypothalamic-pituitary-adrenal axisbrain-derived neurotrophic factor/tyrosine kinase B receptor/cyclic adenosine phosphate reaction element binding protein signaling pathwayhippocampus
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