The Mechanism of Antler Polypeptide Regulating PI3K/AKT Signaling Pathway through SDF-1α/CXCR4 Axis Promotes Angiogenesis on Rats with Lower Extremity Arteriosclerotic Obliterans
Objective:To explore the effect of antler polypeptides on phosphatidylinoinosidine-3-kinase(PI3K)/protein kinase B(AKT)pathway through stromal cell derived factor(SDF-1α)/receptor(CXCR4)axis,and to promote angiogenesis on rats with lower limb arteriosclerotic obliterans(PAD).Methods:The rats model with lower extremity arteriosclerosis obliterans were established by ligation and severing of femoral artery and branches to construct limb ischemia and feeding with high fat diet.One hundred male SD rats were randomly divided into blank group,model group and sham operation group;antler polypeptide low dose group,medium dose antler polypeptide medium dose group,antler polypeptide high dose group,inhibitor LY294002 group,LY294002+antler polypeptide low dose group,LY294002+antler polypeptide medium dose group,LY294002+antler polypeptide high dose group.The blank group,model group and sham group were gavaged equal volume of distilled water.LY294002+antler polypeptide low dose group,LY294002+antler polypeptide medium dose group,LY294002+antler polypeptide high dose group were first injected intraperitoneally and then gavaged with intragastric administration,once a day after modeling,and rats,were executed after 28 days.Flow cytometry was used to detect the proportion of hematopoietic stem cells(CD34+cells)in peripheral blood and bone marrow after administration.The proportion of SDF-1α and PI3K positive cells in gastrocnemius muscle was detected by immunofluorescence assay.Results:The proportion of hematopoietic stem cells(CD34+ cells)in model group was higher than that in blank group(P<0.05).The proportion of positive cells in antler polypeptide low dose group was higher than that in LY294002+antler polypeptide low dose group,the proportion of positive cells in antler polypeptide medium dose group was higher than that in LY294002+antler polypeptide medium dose group,and the proportion of positive cells in antler polypeptide high dose group was higher than that in LY294002+antler polypeptide high dose group(P<0.05).The proportion of positive cell number of SDF-1α and PI3K in administration group was significantly higher than that in model group and inhibition group(P<0.05),the antler polypeptide medium dose group was higher than that in LY294002+antler polypeptide medium dose group group,and the antler polypeptide medium dose group was higher than that in antler polypeptide low dose group group(P<0.05),antler polypeptide high dose group was higher than LY294002+antler polypeptide high dose group(P<0.05).Conclusion:Antler polypeptide affected the PI3K/AKT signaling pathway through SDF-1α/receptor(CXCR4)axis thereby promoting the proliferation and differentiation of endothelial progenitor cells,and facilitating the angiogenesis of PAD.
arteriosclerotic obliterans of lower extremityantler polypeptideendothelial progenitor cellsangiogenesisexperimental study
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维普
