Metabolomics Study on the Intervention of Shugan Jieyu Anshen Formula for Coronary Heart Disease Complicated with Depression
Objective:To explore the mechanism of Shugan Jieyu Anshen Formula for treating coronary heart disease with depression.Methods:Patients with coronary heart disease and depression were randomly divided into treatment group(9 cases,Shugan Jieyu Anshen Formula+Western medicine conventional treatment+psychological counseling treatment)and control group(8 cases,Western medicine conventional treatment+psychological counseling treatment),treated for 1 week.The high-performance liquid chromatography-mass spectrometry(HPLC-MS)was used to detect serum metabolites before and after treatment in two groups,screen for potential differential metabolites,and predict metabolic pathways related to the treatment of coronary heart disease combined with depression with Shugan Jieyu Anshen Formula.Results:Through secondary mass spectrometry analysis,a total of 26 secondary differential metabolites were ultimately obtained in this study.After the intervention of Shugan Jieyu Anshen Formula,compared with the metabolic spectrum of patients with coronary heart disease complicated with depression before intervention,the expression levels of seven metabolites(baicalin,fisetin,N5-L-1-carboxyethyl-L-ornidine,5-hydroxytryptamine,aconite,cysteine sulfate,5-2-hydroxyethyl-4-methylthiazole)were up-regulated after the intervention of traditional Chinese medicine.The expression levels of six metabolites,including N-[(3a,5b,7a)-3-hydroxy-24-oxy-7-(sulfoxide)choleglycan-24-yl]-glycine,L-gibberellin,undecanoic acid,citrulline,nonanoic acid,and styrene oxide were downregulated after traditional Chinese medicine intervention.The Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis showed there were 26 secondary differential metabolites,and ultimate enrichment showed there were 46 metabolic pathways.The four pathways that were significantly enriched include the cAMP signaling pathway,citric acid cycle(TCA cycle),bile secretion,and 5-hydroxytryptamine synaptic pathway.Conclusion:This study reveal what exactly happened in the body after diseases and the intervention by traditional Chinese medicine prescriptions.From the perspective of metabolic end products,it elucidated the mechanism of action of Shugan Jieyu Anshen Formula for treating coronary heart disease with depression,providing a certain reference basis for subsequent experiments and the development of new drugs.
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