Effect of Aβ25-35 on TREM2/NF-κB Signaling Pathway in BV2 Microglia
Objective:To investigate the effects of different concentrations of beta-amyloid peptide 25-35(Aβ25-35)on triggering receptor expressed on myeloidcells 2(TREM2)/nuclear factor-κB(NF-κB)signaling pathway in microglia.Methods:BV2 cells were randomly divided into 5 groups,treated with 0,5,10,20,and 40μmol/L Aβ25-35 for 24 h and 48 h.Cell morphology was observed under microscope,cell proliferation activity was determined by CCK-8 method,TNF-α expression was determined by enzyme-linked immunosorbent assay(ELISA).The mRNA expression of NF-κB p65 and TREM2 were determined by reverse transcription-polymerase chain reaction(RT-PCR).Results:Under the microscope,the cells died in slices after induction by Aβ25-35≥10μmol/L for 48 h.After 24 hours of intervention,the survival rate of BV2 cells treated with different concentrations of Aβ25-35 was>70%.Compared with the control group,the expression of pro-inflammatory cytokines TNF-αand TREM2 mRNA significantly increased in Aβ25-35 20,40μmol/L,and the expression of NF-κB p65 mRNA significantly increased in Aβ25-35 20μmol/L,the difference was statistically significant(P<0.05).Conclusion:The concentration of Aβ25-35 at 20 μmol/L for 24 h can activate microglia to induce inflammation,which may be related to the activation of TREM2/NF-κB signaling pathway.
Alzheimer's diseaseinflammationBV2 cellsbeta-amyloid peptide 25-35,Aβ25-35experimental study
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