摘要
目的:观察艾司氯胺酮对大鼠下肢缺血再灌注(LIR)所诱发的心肌损伤的影响,探索艾司氯胺酮不同给药时间对心肌损伤的影响.方法:将 30只 SD大鼠随机分为假手术组(Sham组)、缺血再灌注组(IR组)、假手术+艾司氯胺酮组(Sham+Esk组)、缺血前艾司氯胺酮+缺血再灌注组(IR+Esk1组)、再灌注前艾司氯胺酮+缺血再灌注组(IR+Esk2组),每组 6只.除假手术组外,其余各组制备下肢缺血再灌注模型(缺血 3h,再灌注 2h).经药物处理后,采用酶联免疫吸附试验(ELISA)法检测各组大鼠血清肌钙蛋白 I(cTnI)、肿瘤坏死因子α(TNF-α)、白细胞介素-6(IL-6)、去甲肾上腺素(NE)含量的变化.结果:相较于 Sham组,IR组血清 cTnI、TNF-α、IL-6、NE含量升高,Sham+Esk组血清 cTnI、TNF-α、IL-6含量降低、NE含量升高(P<0.05).相较于 IR组,IR+Esk1 组和IR+Esk2组血清cTnI、TNF-α、IL-6含量降低,IR+Esk1组血清NE含量降低、IR+Esk2组血清NE含量升高,差异有统计学意义(P<0.05).结论:艾司氯胺酮可能通过抑制炎症反应对大鼠下肢缺血再灌注导致的心肌损伤产生保护作用.
Abstract
Objective:To observe the effects of esketamine on myocardial injury(MI)induced by hindlimb ischemia reperfusion(LIR)in rats,and explore the effects of different administration times of esketamine on myocardial injury.Methods:Thirty SD rats were randomly divided into sham surgery group(Sham group),ischemia reperfusion group(IR group),sham surgery+esketamine group(Sham+Esk group),pre-ischemic esketamine+ischemia-reperfusion group(IR+Esk1 group)and pre-reperfusion esketamine+ischemia-reperfusion group(IR+Esk2 group),with 6 rats in each group.Hindlimb ischemia reperfusion model were prepared in other groups except sham group(ischemia 3 h,reperfusion 2 h).After drug treatment,the contents of troponin I(cTnI),tumor necrosis factorα(TNF-α),interleukin-6(IL-6),and norepinephrine(NE)in serum of each group were measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with Sham group,the contents of serum cTnI,TNF-α,IL-6,and NE contents increased in IR group,while cTnI,TNF-α and IL-6 contents decreased and NE contents increased in Sham+Esk group(P<0.05).Compared with IR group,the contents of serum cTnI,TNF-α and IL-6 contents in IR+Esk1 group and IR+Esk2 group decreased,serum NE content in IR+Esk1 group decreased,and NE in IR+Esk2 group increased,with statistical significance(P<0.05).Conclusion:Esketamine may show a protective effect on myocardial injury induced by hindlimb ischemia reperfusion in rats by inhibiting inflammatory response.
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