The Effect of Panax Quinquefolium L.and Salvia Miltiorrhiza Bunge.on Myocardial Ischemia in Rats after Myocardial Infarction Based on Transcriptomic Sequencing
Objective:To explore the therapeutic targets of of Panax quinquefolium L.and Salvia miltiorrhiza Bunge.on myocardial ischemia in rats after myocardial infarction using transcriptomic sequencing.Methods:The rat model of myocardial infarction was established by ligation of the anterior descending branch of the left coronary artery.The rats with successful modeling were randomly divided into model group(MOD group,equal volume of distilled water),Panax quinquefolium L.and Salvia miltiorrhiza Bunge.low-dose group[XD-L group,Panax quinquefolium L.1.5 g/(kg·d)+Salvia miltiorrhiza Bunge.4.5 g/(kg·d)]and Panax quinquefolium L.and Salvia miltiorrhiza Bunge.high-dose group[XD-H group,Panax quinquefolium L.3 g/(kg·d)+Salvia miltiorrhiza Bunge.9 g/(kg·d)],angiotensin-converting enzyme inhibitors(ACEI)group[Perindopril 0.84 mg/(kg·d)],sham operation rats were used as control group(CON group,equal volume of distilled water),6 rats in each group,the rats were given continuous gavage for 4 weeks.The cardiac function of rats were detected by echocardiography,and hematoxylin-eosin(HE)staining was used to observe the histopathological changes of rat myocardium.The differentially expressed genes(DEGs)of Panax quinquefolium L.and Salvia miltiorrhiza Bunge.were screened by transcriptomic sequencing,and the DEGs were enriched by gene ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG).Results:Panax quinquefolium L.and Salvia miltiorrhiza Bunge.could improve the cardiac function and alleviate the pathological injury of myocardial tissue in rats after myocardial infarction.Transcriptomic sequencing results showed that compared with CON group,12 genes in MOD group were up-regulated and 13 genes were down-regulated.Panax quinquefolium L.and Salvia miltiorrhiza Bunge.switched back 6 genes in MOD group.DEGs was mainly concentrated in tricarboxylic acid(TCA)cycle and phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signaling pathways.lt was related to immune inflammatory reaction,cell apoptosis and angiogenesis.Conclusion:Panax quinquefolium L.and Salvia miltiorrhiza Bunge.could improve the cardiac function of rats after myocardial infarction by up-regulating Wnt4 expression,inhibiting apoptosis and inflammatory response.
myocardial infarctionPanax quinquefolium L.Salvia miltiorrhiza Bunge.transcriptomic sequencingmyocardial ischemiaratsexperimental study
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