参元丹干预氧化三甲胺诱导人脐静脉内皮细胞损伤的机制研究
The Effect of Shenyuandan on the Injury of HUVEC Induced by Trimethylamine Oxide
贾思涵 1尚菊菊 1刘红旭 1李思耐 2李享1
作者信息
- 1. 首都医科大学附属北京中医医院(北京 100010)
- 2. 首都医科大学附属北京中医医院(北京 100010);北京市中医药研究所(北京 100010)
- 折叠
摘要
目的:探讨参元丹对氧化三甲胺(TMAO)诱导人脐静脉内皮细胞(HUVEC)损伤的干预作用及机制.方法:建立 TMAO诱导的 HUVEC细胞损伤模型,将 HUVEC分为正常对照组、模型组(300 μmol/L TMAO)、参元丹低剂量组(300μmol/L TMAO+25μg/mL参元丹)、参元丹中剂量组(300μmol/L TMAO+50μg/mL参元丹)、参元丹高剂量组(300μmol/L TMAO+100μg/mL参元丹).分别检测各组细胞中活性氧(ROS)、丙二醛(MDA)、白细胞介素(IL)-1β含量.结果:TMAO可诱导 HUVEC发生氧化应激反应及炎症反应,参元丹可降低 TMAO损伤细胞内 ROS、MDA水平(P<0.05),同时抑制 IL-1β的表达水平(P<0.05).结论:参元丹可能通过抑制氧化应激反应及炎症反应,减轻 TMAO诱导的内皮细胞损伤.
Abstract
Objective:To investigate the effect of Shenyuandan on trimethylamine oxide(TMAO)induced injury of human umbilical vein endothelial cells(HUVEC).Methods:TMAO induced HUVEC cell damage model was established.HUVEC were divided into normal control group,model group(300 μmol/L TMAO),Shenyuandan low-dose group(300 μmol/L TMAO+25 μg/mL Shenyuandan),Shenyuandan medium-dose group(300μmol/L TMAO+50μg/mL Shenyuandan),and Shenyuandan high-dose group(300μmol/L TMAO+100μg/mL Shenyuandan).The contents of reactive oxygen species(ROS),malondialdehyde(MDA),interleukin(IL)-1βin each group were detected.Results:TMAO could induce oxidative stress and inflammation in HUVEC.Shenyuandan could decrease the ROS and MDA levels in TMAO injured cells(P<0.05),and inhibit the expression level of IL-1β(P<0.05).Conclusion:endothelial cell Shenyuandan may reduce TMAO induced damage by inhibiting oxidative stress and inflammation.
关键词
人脐静脉内皮细胞/氧化应激/氧化三甲胺/参元丹/实验研究Key words
human umbilical vein endothelial cell/oxidative stress/trimethylamine oxide/Shenyuandan/experimental study引用本文复制引用
出版年
2024
NETL
NSTL
万方数据