Mechanism of Chuanxiong-Chishao and its Effective Ingredients on ox-LDL-induced Macrophage Foaming and Hepcidin
Objective:To observe the effect of Chuanxiong-Chishao and its effective ingredients on cell foaming,and to explore its mechanism of action.Methods:The foaming model of THP-1 cells was established by oxidized low density lipoprotein(ox-LDL).The tohoku hospital pediatrics-1(THP-1)cells induced by ox-LDL were treated with Chuanxiong-Chishao and its effective ingredients respectively.The cell foam was analyzed by oil red O staining,and the mRNA expressions of hepcidin(Hep),ferroportin(FPN),ferrimodulin regulatory protein(HJV),hemochromatosis gene(HFE)and transferrin receptor 2(TfR2)were detected by reverse transcription quantitative polymerase chain reaction(RT-qPCR).Results:Oil red O staining showed that the number of red fat drops decreased with the intervention of Chuanxiong-Chishao and its effective ingredients.Compared with the control group,the mRNA expressions of Hep,FPN,HFE and TfR2 increased in the model group,while mRNA expression of HJV decreased.Compared with the model group,Chuanxiong-Chishao and its effective ingredients of the intervention group showed obvious inhibitory effect,and the differences were statistically significant(P＜0.05 or P＜0.01).Conclusion:Chuanxiong-Chishao and its effective ingredients could inhibit the ox-LDL-induced THP-1-derived macrophage foaming,and its mechanism of action was related to the regulation of iron metabolism related gene expression.
Chuanxiong-Chishaomacrophage foamingferroportinligustrazinepaeoniflorinexperimental study
万方数据
维普
