Effect of EEF1A1 on Myocardial Cell Apoptosis and Notch/AKT Transduction Pathway in Rats with Acute Myocardial Infarction
Objective:To explore the effect of homo sapiens eukaryotic translation elongation factor 1 alpha 1(EEF1A1)on myocardial cell apoptosis and Notch/protein kinase B(AKT)transduction pathway in acute myocardial infarction(AMI)rats model.Methods:Thirty male SD rats were randomly divided into sham operation group,model group,and intervention group,with 10 rats in each group.The rats in model group and intervention group AMI rat model,established by ligating the left anterior descending coronary artery,while the control group only underwent surgical threading without ligation.Twenty-four hours after establishment,the rats in intervention group were injected with EEF1A1 adenovirus in situ in the myocardial infarction area,and the rats in model group and control group were injected with the same volume of normal saline.After 72 hours of intervention,the cardiao function was measured by color Doppler ultrasound,the apoptosis of cardiao myocytes was evaluated by Terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling(TUNEL)staining method,the infarct area was evaluated by 2,3,5-triphenyltetrazolium chloride(TCC)staining method,the expression of EEF1A1 mRNA was measured by real-time quantitative PCR method,and the expression of EEF1A1 protein and Notch/AKT signal pathway related protein in myocardial tissue was detected by Western Blot.Results:Before intervention,left ventricular ejection fraction(LVEF)in the model group and intervention group was significantly lower than that in the sham operation group,while left ventricular end-diastolic dimension(LVEDD)and left ventricular end-systolic diameter(LVESD)were significantly higher than those in sham operation group(P＜0.05).After 3 days of intervention,LVEDD and LVESD in intervention group significantly decreased,while LVEF significantly increased,and LVEDD and LVESD in model group significantly increased,while LVEF significantly decreased,and the differences were statistically significant(P＜0.05).The expression levels of myocardial EEF1A1 protein and mRNA in intervention group were higher than those in sham operation group and model group(P＜0.05),and the expression levels of myocardial EEF1A1 protein and mRNA in model group were higher than those in sham operation group(P＜0.05).The myocardial infarct size in intervention group was significantly lower than that in model group(P＜0.05).The apoptosis rate of myocardial cells in intervention group was higher than that in sham operation group(P＜0.05),and lower than that in model group(P＜0.05).The levels of Notch1,Hes1,and phosphorylated AKT(p-AKT)/AKT-related proteins in intervention group were higher than those in model group and sham operation group(P＜0.05).The levels of Notch1,Hes1,and p-AKT/AKT-related proteins in model group were higher than those in sham operation group(P＜0.05).Conclusion:EEF1A1 can inhibit myocardial cell injury and apoptosis,reduce myocardial infarction area,and improve cardiac function in AMI rats.Its mechanism may be related to the up-regulation of Notch/AKT transduction pathway.
acute myocardial infarctioneukaryotic translation elongation factor 1 alpha1cell apoptosiscardiac functionNotch/protein kinase B transduction pathwayexperimental study
万方数据
维普
