Objective:To investigate the effect of poric acid on oxidized low densitylipoprotein(ox-LDL)-induced apoptosis of vascular endothelial cells by regulating miR-145-5p/Krüppel-like factor 5(KLF5)axis.Methods:Human umbilical vein endothelial cells(HUVEC)were grouped into control group,ox-LDL group,ox-LDL+poric acid group,ox-LDL+poric acid+inhibitor-NC group,and ox-LDL+poric acid+miR-145-5p inhibitor group.After grouping,CCK-8 method and Edu staining were applied to detect cell proliferation.The levels of interleukin(IL)-1βand tumor necrosis factor-α(TNF-α)were detected by enzyme linked immunosorbent assay(ELISA)kits.The contents of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione(GSH)were analyzed with the kit.The expression levels of miR-145-5p and KLF5 mRNA were detected by reverse transcriptase-polymerase chain reaction(qRT-PCR).The apoptosis rate was detected by flow cytometry.Western Blot was applied to detect the expression of KLF5,Bax and Bcl-2 proteins in the cells.The relationship between miR-145-5p and KLF5 was verified by double luciferase reporter gene experiment.Results:Compared with the control group,the HUVEC cell activity,proliferation rate,SOD and GSH,the expression of miR-145-5p,and the protein expression level of Bcl-2 in ox-LDL group decreased obviously,the IL-1β,TNF-α,MDA,apoptosis rate,the expression of KLF5 mRNA,the protein expression levels of KLF5 and Bax increased obviously(P<0.05).Compared with ox-LDL group,the HUVEC cell activity,proliferation rate,SOD and GSH,the expression of miR-145-5p,and the protein expression level of Bcl-2 in ox-LDL+poric acid group and ox-LDL+poric acid+inhibitor-NC group increased obviously,the IL-1β,TNF-α,MDA,apoptosis rate,the expression of KLF5 mRNA,the protein expression levels of KLF5 and Bax decreased obviously(P<0.05).Compared with ox-LDL+poric acid+inhibitor-NC group,the HUVEC cell activity,proliferation rate,SOD and GSH,the expression of miR-145-5p,and the protein expression level of Bcl-2 in ox-LDL+poric acid+miR-145-5p inhibitor group decreased obviously,the IL-1β,TNF-α,MDA,apoptosis rate,the expression of KLF5 mRNA,the protein expression levels of KLF5 and Bax increased obviously(P<0.05).The double luciferase reporter gene experiment verified that there was a targeted regulatory relationship between miR-145-5p and KLF5.Conclusion:Poric acid can reduce the apoptosis of vascular endothelial cells induced by ox-LDL,and its mechanism may be related to the regulation of miR-145-5p/KLF5 axis.
vascular endothelial cellsapoptosisporic acidmiR-145-5papoptosis,KLF5oxidized low density lipoproteinexperimental study
维普
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