Objective:To explore the causal relationship between vitamin D and coronary heart disease(CHD).Methods:Independent single nucleotide polymorphism(snp) loci strongly associated with vitamin D and CHD were collected from a number of extensive genome-wide association studies(GWAS) as instrumental variables.The inverse variance weighting(IVW),weighted median and MR-Egger method were used to estimate Mendelian randomization.Cochran's Q test,MR-Egger intercept test,MR-PRESSO test,MR Steiger test,leave-one-out analysis,and funnel plot were used for sensitivity analysis.Results:A total of 69 SNPS of vitamin D were screened.IVW analysis showed a causal relationship between vitamin D and CHD(OR=0.994,95％CI 0.988-0.999,P=0.0476).Cochran's Q showed Q=179.7898,P=1.76e-12,indicating that there was no heterogeneity among the SNP in the analysis.The MR-Egger intercept was P=0.7461144,indicating that there was no genetic pleiotropy in the SNP obtained from the screening.MR-PRESSO did not find any outlier SNP.Leave-one-out sensitivity analysis showed that causal estimation would not be affected by the effect of certain SNP.Reverse Mendelian randomization analysis IVW analysis,OR=0.416,95％CI(0.064-2.688),P=0.35705610,Cochran's Q shows Q=66.90252,P=1.022131e-13,and MR-Egger intercept (P=0.85615),indicated that vitamin D and CHD did not support reverse causality.Conclusion:This Mendelian randomization study demonstrates some causal association between vitamin D and CHD,but reverse causality is unreliable and to helpful to understanding some the mechanisms that vitamin D protects CHD.
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