Objective:To verify the intervention effect of Professor Guo Weiqin Yiqi Xifei Decoction on doxorubicin cardiotoxicity by network pharmacology.Methods:Rat model of doxorubicin induced cardiotoxicity were constructed.Cardiac troponin T ( cTnT) was detected by enzyme-linked immunosorbent assay(ELISA) to evaluate myocardial injury.Hematoxylin-eosin(HE) and Masson staining were used to observe cardiac inflammation and fibrosis.Potential drug related targets in Yiqi Xifei Decoction were obtained through the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform( TCMSP),and the doxorubicin cardiotoxic targets screened from human Menelian Genetics online database ( OMIM),drug database ( DrugBank) and gene card database (GeneCards).The intersection target protein interaction network diagram was constructed based on STRING database.According to Cytoscape 3.10.1 software,the disease target network,which was the key active ingredient of Yiqi Xifei Decoction,was constructed,and the core targets were screened.The gene ontology(GO) and the Kyoto Encyclopedia of Genes and Genomes(KEGG) were enriched for the intersection targets of drugs and diseases using Metascape platform,and the possible signal pathways involved in the treatment of doxorubicin induced cardiotoxicity by Yiqi Xifei Decoction were screened.Results:The results of animal experiments showed that Yiqi Xifei Decoction could reduce inflammatory cell infiltration and collagen fiber deposition in myocardial tissue,reduce cTnT level,significantly improve myocardial injury,and effectively intervene doxorubicin induced cardiotoxicity.The network pharmacological results showed that there were 130 chemical components of 10 drugs in Yiqi Xifei Decoction,which acted on 1216 gene targets.Huangqi(Radix Scutellariae),Tinglizi(Lyocarpa),Sangbaipi(Cortex Mori),Honghua(Carthamus tinctorius),and Cheqianzi (Plantain seed) were the core drugs.Quercetin,kaempferol,luteolin,baicalin and tanshinone ⅡA were the main compounds.Protein kinase B(AKT) 1,tumor suppressor protein(TP53),transcription activator 3(STAT3),and tumor necrosis factor(TNF) were the key targets.Doxorubicin induced cardiotoxicity were mainly interfered by the interaction of lipids with atherosclerosis,TNF signaling pathway and necrosis pathways.Conclusion:The action mechanism of Yiqi Xifei Decoction in the intervention of doxorubicin induced cardiotoxicity involves multiple components,multiple targets and multiple pathways.
维普
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